Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275

Zennah C. Kosgey; Erena A. Edae; Ruth Dill-Macky; Yue Jin; Worku Denbel Bulbula; Ashenafi Gemechu; Godwin Macharia; Sridhar Bhavani; Mandeep S. Randhawa; Matthew N. Rouse

doi:10.3389/fpls.2020.609659

Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275

Zennah C. Kosgey, Erena A. Edae, Ruth Dill-Macky, Yue Jin, Worku Denbel Bulbula, Ashenafi Gemechu, Godwin Macharia, Sridhar Bhavani, Mandeep S. Randhawa, Matthew N. Rouse

Plant Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.

Original language	English (US)
Article number	609659
Journal	Frontiers in Plant Science
Volume	11
DOIs	https://doi.org/10.3389/fpls.2020.609659
State	Published - Jan 12 2021

Bibliographical note

Funding Information:
We acknowledge Sam Stoxen, Samantha Armintrout, and Greta Ciessau for their support in planting and maintaining field and greenhouse experiments, for technical help in the laboratory, and the assistance with manual SNP calling of 90K SNP data. We also extend our gratitude to the technical staff of the Kenya Agricultural and Livestock Research Organization (KALRO), Njoro, Kenya, and the Debre Zeit Agricultural Research Centre, Ethiopia, for their assistance in establishing and maintaining field experiments. Funding. We acknowledge the financial support provided by the United States Agency for International Development as part of the Feed the Future initiative under the CGIAR Fund, award number BFS-G-11-00002, and the predecessor fund the Food Security and Crisis Mitigation II grant, award number EEM-G-00-04-00013 and the USDA-ARS National Plant Disease Recovery System.

Funding Information:
We acknowledge the financial support provided by the United States Agency for International Development as part of the Feed the Future initiative under the CGIAR Fund, award number BFS-G-11-00002, and the predecessor fund the Food Security and Crisis Mitigation II grant, award number EEM-G-00-04-00013 and the USDA-ARS National Plant Disease Recovery System.

Publisher Copyright:
© Copyright © 2021 Kosgey, Edae, Dill-Macky, Jin, Bulbula, Gemechu, Macharia, Bhavani, Randhawa and Rouse.

Keywords

Puccinia graminis f. sp. tritici
QTL
Triticum aestivum
Ug99
disease resistance
genetics
stem rust

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fpls.2020.609659

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Cite this

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title = "Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275",

abstract = "Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.",

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author = "Kosgey, {Zennah C.} and Edae, {Erena A.} and Ruth Dill-Macky and Yue Jin and Bulbula, {Worku Denbel} and Ashenafi Gemechu and Godwin Macharia and Sridhar Bhavani and Randhawa, {Mandeep S.} and Rouse, {Matthew N.}",

note = "Funding Information: We acknowledge Sam Stoxen, Samantha Armintrout, and Greta Ciessau for their support in planting and maintaining field and greenhouse experiments, for technical help in the laboratory, and the assistance with manual SNP calling of 90K SNP data. We also extend our gratitude to the technical staff of the Kenya Agricultural and Livestock Research Organization (KALRO), Njoro, Kenya, and the Debre Zeit Agricultural Research Centre, Ethiopia, for their assistance in establishing and maintaining field experiments. Funding. We acknowledge the financial support provided by the United States Agency for International Development as part of the Feed the Future initiative under the CGIAR Fund, award number BFS-G-11-00002, and the predecessor fund the Food Security and Crisis Mitigation II grant, award number EEM-G-00-04-00013 and the USDA-ARS National Plant Disease Recovery System. Funding Information: We acknowledge the financial support provided by the United States Agency for International Development as part of the Feed the Future initiative under the CGIAR Fund, award number BFS-G-11-00002, and the predecessor fund the Food Security and Crisis Mitigation II grant, award number EEM-G-00-04-00013 and the USDA-ARS National Plant Disease Recovery System. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Kosgey, Edae, Dill-Macky, Jin, Bulbula, Gemechu, Macharia, Bhavani, Randhawa and Rouse.",

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AU - Edae, Erena A.

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AU - Jin, Yue

AU - Bulbula, Worku Denbel

AU - Gemechu, Ashenafi

AU - Macharia, Godwin

AU - Bhavani, Sridhar

AU - Randhawa, Mandeep S.

AU - Rouse, Matthew N.

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N2 - Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.

AB - Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.

KW - Puccinia graminis f. sp. tritici

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KW - Triticum aestivum

KW - Ug99

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KW - genetics

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Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275

Abstract

Bibliographical note

Keywords

UN SDGs

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