Measurement of deubiquitinating enzyme activity via a suicidal HA-UB-VS probe

Colleen Rivard; Martina Bazzaro

doi:10.1007/978-1-4939-2013-6_14

Measurement of deubiquitinating enzyme activity via a suicidal HA-UB-VS probe

Colleen Rivard, Martina Bazzaro

Obstetrics, Gynecology and Women's Health - Oncology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

Original language	English (US)
Pages (from-to)	193-200
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1249
DOIs	https://doi.org/10.1007/978-1-4939-2013-6_14
State	Published - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Cervical cancer
Cysteine proteases
DUB
Deubiquitinating enzymes
HA-Ub-VS probe
Metalloproteases
P53
Protease inhibitors
Proteases
Proteasome
Ubiquitin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Measurement of deubiquitinating enzyme activity via a suicidal HA-UB-VS probe",

abstract = "Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.",

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author = "Colleen Rivard and Martina Bazzaro",

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doi = "10.1007/978-1-4939-2013-6_14",

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volume = "1249",

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AU - Bazzaro, Martina

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KW - Cysteine proteases

KW - DUB

KW - Deubiquitinating enzymes

KW - HA-Ub-VS probe

KW - Metalloproteases

KW - P53

KW - Protease inhibitors

KW - Proteases

KW - Proteasome

KW - Ubiquitin

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Measurement of deubiquitinating enzyme activity via a suicidal HA-UB-VS probe

Abstract

Bibliographical note

Keywords

UN SDGs

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