@inproceedings{69613f8c65314b88a5c05c60b3c8f480,
title = "Measuring bacterial adaptation dynamics at single-cell level using a microfluidic chemostat and time-lapse fluorescence microscopy",
abstract = "We report the operation of a microchemostat device for monitoring the dynamic response of single E. coli bacterial cells to nutritional downshift using time-lapse fluorescence microscopy. We observed two E. coli strains containing different promoter-reporter gene constructs. Both strains have similar adaptation dynamics for cell size rearrangement. However, the GFP expression dynamics of these two strains are distinctly different. The mean GFP concentration in the P1 strain cells with a ribosomal RNA promoter changes rapidly during nutritional downshift, while that in the P5 strain cells with a constitutive promoter remains relatively stable.",
keywords = "Bacterial adaptation, Microchemostat, Single-cell dynamics",
author = "Zhicheng Long and Anne Olliver and Elisa Brambilla and Bianca Sclavi and Lagomarsino, {Marco Cosentino} and Dorfman, {Kevin D.}",
year = "2014",
month = jan,
day = "1",
language = "English (US)",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "834--836",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
}