Abstract
An extensive culture-dependent and -independent study was conducted to identify microorganisms contributing to the biogeochemical cycling of manganese (Mn) in Ashumet Pond, a freshwater pond in Massachusetts currently undergoing remediation. A variety of bacteria (including Gamma-, Beta-, and Alpha-proteobacteria, Firmicutes, and Bacteroides) and Ascoymete fungi were isolated from the pond that promote Mn(II) oxidation and subsequent formation of Mn(III/IV) oxide minerals. Targeted-amplicon pyrosequencing of the bacterial and fungal communities associated with Mn oxide-encrusted samples show a highly diverse microbial community, of which the cultured phylotypes represent a minor proportion. This suggests a larger community, not identified through culturing, contributes to Mn oxide formation within the Pond. © 2014
| Original language | English (US) |
|---|---|
| Pages (from-to) | 605-616 |
| Number of pages | 12 |
| Journal | Geomicrobiology Journal |
| Volume | 31 |
| Issue number | 7 |
| DOIs | |
| State | Published - Aug 2014 |
| Externally published | Yes |
Bibliographical note
Funding Information:This research was sponsored in part by by the National Science Foundation, Grant Number EAR-0846715, awarded to CMH as well as funding through the Grants program awarded to CMS.
Keywords
- biogeochemical cycling
- biomineralization
- bioremediation
- community structure
- molecular ecology
