Microsatellites from the charcoal rot fungus (Macrophomina phaseolina)

Richard E. Baird; Phillip A. Wadl; Xinwang Wang; Denita H. Johnson; Timothy A. Rinehart; Hamed K. Abbas; Thomas Shier; Robert N. Trigiano

doi:10.1111/j.1755-0998.2008.02511.x

Microsatellites from the charcoal rot fungus (Macrophomina phaseolina)

Richard E. Baird, Phillip A. Wadl, Xinwang Wang, Denita H. Johnson, Timothy A. Rinehart, Hamed K. Abbas, Thomas Shier, Robert N. Trigiano

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.

Original language	English (US)
Pages (from-to)	946-948
Number of pages	3
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02511.x
State	Published - May 2009

Keywords

Genomic library
PCR
SSR enrichment

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abstract = "Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.",

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AU - Baird, Richard E.

AU - Wadl, Phillip A.

AU - Wang, Xinwang

AU - Johnson, Denita H.

AU - Rinehart, Timothy A.

AU - Abbas, Hamed K.

AU - Shier, Thomas

AU - Trigiano, Robert N.

PY - 2009/5

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AB - Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.

KW - Genomic library

KW - PCR

KW - SSR enrichment

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Microsatellites from the charcoal rot fungus (Macrophomina phaseolina)

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