Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)

Rita Baraldi; Kai Hsien Chen; Jerry D. Cohen

doi:10.1016/S0021-9673(00)94477-7

Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)

Rita Baraldi, Kai Hsien Chen, Jerry D. Cohen

Horticultural Science

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Abstract

A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C₁₈ column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. ¹³C₆indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.

Original language	English (US)
Pages (from-to)	301-306
Number of pages	6
Journal	Journal of Chromatography A
Volume	442
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(00)94477-7
State	Published - 1988

Bibliographical note

Funding Information:
l Part of this work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the USDA/ARS and the University of Maryland and, in addition, this work was funded by United States National Science Foundation Metabolic Biology grant DMB-8617171. ** Current address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205, U.S.A.

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title = "Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)",

abstract = "A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.",

author = "Rita Baraldi and Chen, {Kai Hsien} and Cohen, {Jerry D.}",

note = "Funding Information: l Part of this work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the USDA/ARS and the University of Maryland and, in addition, this work was funded by United States National Science Foundation Metabolic Biology grant DMB-8617171. ** Current address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205, U.S.A.",

year = "1988",

doi = "10.1016/S0021-9673(00)94477-7",

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AU - Baraldi, Rita

AU - Chen, Kai Hsien

AU - Cohen, Jerry D.

N1 - Funding Information: l Part of this work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the USDA/ARS and the University of Maryland and, in addition, this work was funded by United States National Science Foundation Metabolic Biology grant DMB-8617171. ** Current address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205, U.S.A.

PY - 1988

Y1 - 1988

N2 - A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.

AB - A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.

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Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)

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