Abstract
Purpose of Review: The introduction of MinION whole-genome sequencing technology greatly increased and simplified complete genome sequencing in various fields of science across the globe. Sequences have been generated from complex organisms to microorganisms and are stored in genome databases that are readily accessible by researchers. Various new software for genome analysis, along with upgrades to older software packages, are being generated. New protocols are also being validated that enable WGS technology to be rapidly and increasingly used for sequencing in field settings. Recent Findings: MinION WGS technology has been implemented in developed countries due to its advantages: portability, real-time analysis, and lower cost compared to other sequencing technologies. While these same advantages are critical in developing countries, MinION WGS technology is still under-utilized in resource-limited settings. Summary: In this review, we look at the applications, advantages, challenges, and opportunities of using MinION WGS in resource-limited settings.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 52-59 |
| Number of pages | 8 |
| Journal | Current Clinical Microbiology Reports |
| Volume | 9 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2022 |
Bibliographical note
Funding Information:This study was supported by the National Institute of Neurological Disorders and Stroke (NINDS) and the Fogarty International Center (FIC) grant no. R01NS118538.
Funding Information:
We appreciate the support and guidance provided by the Genomics and Immunology Group—a collaborative forum to discuss genomic and immunology research related to cryptococcosis that includes researchers at Mbarara University, Makerere University, and the University of Minnesota.
Publisher Copyright:
© 2022, The Author(s).
Keywords
- Flow cells
- MinION
- Sequencing
- Whole-genome
PubMed: MeSH publication types
- Journal Article
- Review
