TY - JOUR
T1 - Minus-end-directed kinesin-14 motors align antiparallel microtubules to control metaphase spindle length
AU - Hepperla, Austin J.
AU - Willey, Patrick T.
AU - Coombes, Courtney E.
AU - Schuster, Breanna M.
AU - Gerami-Nejad, Maryam
AU - McClellan, Mark
AU - Mukherjee, Soumya
AU - Fox, Janet
AU - Winey, Mark
AU - Odde, David J.
AU - O'Toole, Eileen
AU - Gardner, Melissa K.
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/10/13
Y1 - 2014/10/13
N2 - During cell division, a microtubule-based mitotic spindle mediates the faithful segregation of duplicated chromosomes into daughter cells. Proper length control of the metaphase mitotic spindle is critical to this process and is thought to be achieved through a mechanism in which spindle pole separation forces from plus-end-directed motors are balanced by forces from minus-end-directed motors that pull spindle poles together. However, in contrast to this model, metaphase mitotic spindles with inactive kinesin-14minus-end-directed motors often have shorter spindle lengths, along with poorly aligned spindle microtubules. A mechanistic explanation for this paradox is unknown. Using computational modeling, invitro reconstitution, live-cell fluorescence microscopy, and electron microscopy, we now find that the budding yeast kinesin-14 molecular motor Kar3-Cik1 can efficiently align spindle microtubules along the spindle axis. This then allows plus-end-directed kinesin-5 motors to efficiently exert the outward microtubule sliding forces needed for proper spindle bipolarity.
AB - During cell division, a microtubule-based mitotic spindle mediates the faithful segregation of duplicated chromosomes into daughter cells. Proper length control of the metaphase mitotic spindle is critical to this process and is thought to be achieved through a mechanism in which spindle pole separation forces from plus-end-directed motors are balanced by forces from minus-end-directed motors that pull spindle poles together. However, in contrast to this model, metaphase mitotic spindles with inactive kinesin-14minus-end-directed motors often have shorter spindle lengths, along with poorly aligned spindle microtubules. A mechanistic explanation for this paradox is unknown. Using computational modeling, invitro reconstitution, live-cell fluorescence microscopy, and electron microscopy, we now find that the budding yeast kinesin-14 molecular motor Kar3-Cik1 can efficiently align spindle microtubules along the spindle axis. This then allows plus-end-directed kinesin-5 motors to efficiently exert the outward microtubule sliding forces needed for proper spindle bipolarity.
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U2 - 10.1016/j.devcel.2014.07.023
DO - 10.1016/j.devcel.2014.07.023
M3 - Article
C2 - 25313961
AN - SCOPUS:84907987540
SN - 1534-5807
VL - 31
SP - 61
EP - 72
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -