Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass

Nongmaithem Debeni Devi; Xiao Sun; Lingkan Ding; Vaibhav V. Goud; Bo Hu

doi:10.1016/j.jclepro.2022.132834

Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass

Nongmaithem Debeni Devi, Xiao Sun, Lingkan Ding, Vaibhav V. Goud, Bo Hu

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Availability of cost-effective nutrients for the commercial cultivation of microalgae is one of the major challenges. The present study investigated the feasibility of primary municipal wastewater (PMWW) collected from a local wastewater treatment plant in Minnesota, USA as an alternative to fresh-water microalgae growth media towards high-value bioenergy feedstock production. The novel strain Scenedesmus sp. DDVG I was cultivated in the PMWW under heterotrophic and mixotrophic modes in 250 mL Erlenmeyer flasks. The optimized cultivation mode was further scaled up to the 3-L bubbled bioreactor. The study confirmed the wastewater as a potential growth medium while Scenedesmus sp. DDVG I showed superior biomass productivity (0.069 g/L d), lipid yield (22.5%), and FAME content (22.04% in dry cell weight) over 10 days in the mixotrophic mode. The biomass also showed high essential amino acid content (159.8 mg/g DCW). The corresponding values for bioremediation efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) by Scenedesmus sp. were 75.6% and 99.8% respectively. Meanwhile, ammonia nitrogen (NH₃–N) and total phosphorus (TP) were removed at up to 100% removal efficiencies. Analyses of fatty acid profile and different parameters that were required for biodiesel characterization revealed the high-quality nature of the Scenedesmus-derived oil. Overall, the study concluded effective bioremediation of municipal wastewater by Scenedesmus sp. DDVG I with the recovery of valuable resources from the biomass.

Original language	English (US)
Article number	132834
Journal	Journal of Cleaner Production
Volume	365
DOIs	https://doi.org/10.1016/j.jclepro.2022.132834
State	Published - Sep 10 2022

Bibliographical note

Funding Information:
The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota, the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020]

Funding Information:
The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota , the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702 ). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020]

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

Bioremediation
Heterotrophic
Microalgae
Mixotrophic
Scenedesmus sp.
Wastewater treatment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass",

abstract = "Availability of cost-effective nutrients for the commercial cultivation of microalgae is one of the major challenges. The present study investigated the feasibility of primary municipal wastewater (PMWW) collected from a local wastewater treatment plant in Minnesota, USA as an alternative to fresh-water microalgae growth media towards high-value bioenergy feedstock production. The novel strain Scenedesmus sp. DDVG I was cultivated in the PMWW under heterotrophic and mixotrophic modes in 250 mL Erlenmeyer flasks. The optimized cultivation mode was further scaled up to the 3-L bubbled bioreactor. The study confirmed the wastewater as a potential growth medium while Scenedesmus sp. DDVG I showed superior biomass productivity (0.069 g/L d), lipid yield (22.5%), and FAME content (22.04% in dry cell weight) over 10 days in the mixotrophic mode. The biomass also showed high essential amino acid content (159.8 mg/g DCW). The corresponding values for bioremediation efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) by Scenedesmus sp. were 75.6% and 99.8% respectively. Meanwhile, ammonia nitrogen (NH3–N) and total phosphorus (TP) were removed at up to 100% removal efficiencies. Analyses of fatty acid profile and different parameters that were required for biodiesel characterization revealed the high-quality nature of the Scenedesmus-derived oil. Overall, the study concluded effective bioremediation of municipal wastewater by Scenedesmus sp. DDVG I with the recovery of valuable resources from the biomass.",

keywords = "Bioremediation, Heterotrophic, Microalgae, Mixotrophic, Scenedesmus sp., Wastewater treatment",

author = "Devi, {Nongmaithem Debeni} and Xiao Sun and Lingkan Ding and Goud, {Vaibhav V.} and Bo Hu",

note = "Funding Information: The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota, the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020] Funding Information: The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota , the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702 ). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020] Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

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AU - Devi, Nongmaithem Debeni

AU - Sun, Xiao

AU - Ding, Lingkan

AU - Goud, Vaibhav V.

AU - Hu, Bo

N1 - Funding Information: The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota, the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020] Funding Information: The authors would like to thank the Department of Bioproducts and Biosystems Engineering, University of Minnesota , the USA for providing research laboratory facilities and the National Science Foundation for financial support (Award No. 1804702 ). Also, a part of this work was under Bioenergy-Awards for Cutting Edge Research (B-ACER) program [Award Letter No. IUSSTF B-ACER 2019 Internship/5/Nongmaithem Debeni Devi, January 03, 2020] Publisher Copyright: © 2022 Elsevier Ltd

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N2 - Availability of cost-effective nutrients for the commercial cultivation of microalgae is one of the major challenges. The present study investigated the feasibility of primary municipal wastewater (PMWW) collected from a local wastewater treatment plant in Minnesota, USA as an alternative to fresh-water microalgae growth media towards high-value bioenergy feedstock production. The novel strain Scenedesmus sp. DDVG I was cultivated in the PMWW under heterotrophic and mixotrophic modes in 250 mL Erlenmeyer flasks. The optimized cultivation mode was further scaled up to the 3-L bubbled bioreactor. The study confirmed the wastewater as a potential growth medium while Scenedesmus sp. DDVG I showed superior biomass productivity (0.069 g/L d), lipid yield (22.5%), and FAME content (22.04% in dry cell weight) over 10 days in the mixotrophic mode. The biomass also showed high essential amino acid content (159.8 mg/g DCW). The corresponding values for bioremediation efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) by Scenedesmus sp. were 75.6% and 99.8% respectively. Meanwhile, ammonia nitrogen (NH3–N) and total phosphorus (TP) were removed at up to 100% removal efficiencies. Analyses of fatty acid profile and different parameters that were required for biodiesel characterization revealed the high-quality nature of the Scenedesmus-derived oil. Overall, the study concluded effective bioremediation of municipal wastewater by Scenedesmus sp. DDVG I with the recovery of valuable resources from the biomass.

AB - Availability of cost-effective nutrients for the commercial cultivation of microalgae is one of the major challenges. The present study investigated the feasibility of primary municipal wastewater (PMWW) collected from a local wastewater treatment plant in Minnesota, USA as an alternative to fresh-water microalgae growth media towards high-value bioenergy feedstock production. The novel strain Scenedesmus sp. DDVG I was cultivated in the PMWW under heterotrophic and mixotrophic modes in 250 mL Erlenmeyer flasks. The optimized cultivation mode was further scaled up to the 3-L bubbled bioreactor. The study confirmed the wastewater as a potential growth medium while Scenedesmus sp. DDVG I showed superior biomass productivity (0.069 g/L d), lipid yield (22.5%), and FAME content (22.04% in dry cell weight) over 10 days in the mixotrophic mode. The biomass also showed high essential amino acid content (159.8 mg/g DCW). The corresponding values for bioremediation efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) by Scenedesmus sp. were 75.6% and 99.8% respectively. Meanwhile, ammonia nitrogen (NH3–N) and total phosphorus (TP) were removed at up to 100% removal efficiencies. Analyses of fatty acid profile and different parameters that were required for biodiesel characterization revealed the high-quality nature of the Scenedesmus-derived oil. Overall, the study concluded effective bioremediation of municipal wastewater by Scenedesmus sp. DDVG I with the recovery of valuable resources from the biomass.

KW - Bioremediation

KW - Heterotrophic

KW - Microalgae

KW - Mixotrophic

KW - Scenedesmus sp.

KW - Wastewater treatment

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Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass

Abstract

Bibliographical note

Keywords

UN SDGs

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