TY - JOUR
T1 - Molecular and biological characterization of a new isolate of guinea pig cytomegalovirus
AU - Schleiss, Mark R
AU - McAllister, Shane C
AU - Armien, Anibal G
AU - Hernandez-Alvarado, Nelmary
AU - Fernandez Alarcon, Claudia L
AU - Zabeli, Jason C.
AU - Ramaraj, Thiruvarangan
AU - Crow, John A.
AU - McVoy, Michael A.
PY - 2014
Y1 - 2014
N2 - Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland-adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
AB - Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland-adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
KW - CMV immune evasion
KW - Congenital CMV vaccines
KW - Congenital cytomegalovirus infection
KW - Cytomegalovirus strain variation
KW - Guinea pig cytomegalovirus
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U2 - 10.3390/v6020448
DO - 10.3390/v6020448
M3 - Article
C2 - 24473341
AN - SCOPUS:84893280061
SN - 1999-4915
VL - 6
SP - 448
EP - 475
JO - Viruses
JF - Viruses
IS - 2
ER -