Abstract
Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 405-414 |
| Number of pages | 10 |
| DOIs | |
| State | Published - 2022 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2303 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
Keywords
- Drosophila melanogaster
- FLP-Out
- GAL4/UAS
- HSPG
- MARCM
- TARGET
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural