TY - JOUR
T1 - Mu opioid receptor from the zebrafish exhibits functional characteristics as those of mammalian mu opioid receptor
AU - De Velasco, Ezequiel Marron Fdez
AU - Law, Ping Y.
AU - Rodríguez, Raquel E.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - The functional characterization of the mu opioid receptor from the zebrafish (zfMOR) is reported here. After transfection in HEK-293 cell line, using both peptidergic and nonpeptidergic opioid ligands in the competition and saturation-binding experiments, in addition to the functional assays of 35S-GTPγS-binding assays and intracellular 3′-5′- cyclic adenosine monophosphate (cAMP) level determinations, we demonstrate that zfMOR exhibits a pharmacological profile similar to that of the mammalian MOR. Besides, the internalization process of zfMOR after opiate agonist treatment (morphine, DAMGO, etorphine) resembles the pattern observed for its mammalian counterpart. These similarities suggest that the zebrafish is a good model for the study of the opioid effects in development.
AB - The functional characterization of the mu opioid receptor from the zebrafish (zfMOR) is reported here. After transfection in HEK-293 cell line, using both peptidergic and nonpeptidergic opioid ligands in the competition and saturation-binding experiments, in addition to the functional assays of 35S-GTPγS-binding assays and intracellular 3′-5′- cyclic adenosine monophosphate (cAMP) level determinations, we demonstrate that zfMOR exhibits a pharmacological profile similar to that of the mammalian MOR. Besides, the internalization process of zfMOR after opiate agonist treatment (morphine, DAMGO, etorphine) resembles the pattern observed for its mammalian counterpart. These similarities suggest that the zebrafish is a good model for the study of the opioid effects in development.
UR - http://www.scopus.com/inward/record.url?scp=70349337209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349337209&partnerID=8YFLogxK
U2 - 10.1089/zeb.2009.0594
DO - 10.1089/zeb.2009.0594
M3 - Article
C2 - 19761379
AN - SCOPUS:70349337209
SN - 1545-8547
VL - 6
SP - 259
EP - 268
JO - Zebrafish
JF - Zebrafish
IS - 3
ER -
