Myogenic progenitor specification from pluripotent stem cells

Alessandro Magli; Rita R.C. Perlingeiro

doi:10.1016/j.semcdb.2017.10.031

Myogenic progenitor specification from pluripotent stem cells

Alessandro Magli, Rita R.C. Perlingeiro

Medicine - Cardiology Division

Research output: Contribution to journal › Review article › peer-review

26 Scopus citations

Abstract

Pluripotent stem cells represent important tools for both basic and translational science as they enable to study mechanisms of development, model diseases in vitro and provide a potential source of tissue-specific progenitors for cell therapy. Concomitantly with the increasing knowledge of the molecular mechanisms behind activation of the skeletal myogenic program during embryonic development, novel findings in the stem cell field provided the opportunity to begin recapitulating in vitro the events occurring during specification of the myogenic lineage. In this review, we will provide a perspective of the molecular mechanisms responsible for skeletal myogenic commitment in the embryo and how this knowledge was instrumental for specifying this lineage from pluripotent stem cells. In addition, we will discuss the current limitations for properly recapitulating skeletal myogenesis in the petri dish, and we will provide insights about future applications of pluripotent stem cell-derived myogenic cells.

Original language	English (US)
Pages (from-to)	87-98
Number of pages	12
Journal	Seminars in Cell and Developmental Biology
Volume	72
DOIs	https://doi.org/10.1016/j.semcdb.2017.10.031
State	Published - Dec 2017

Bibliographical note

Funding Information:
We thank all the investigators in the skeletal muscle and developmental biology fields for their important contributions and, we apologize for those reports that were not included in this review due to space limitations. RCRP is supported by NIH grants R01 AR055299 and R01 AR071439, the Greg Marzolf Jr Foundation, ADVault, Inc and MyDirectives.com. AM thanks the support from Regenerative Medicine Minnesota.

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

BMP inhibitor
Cell therapy
Disease modeling
ES cells
GSK3 inhibitor
Mesoderm specification
Muscle development
Muscle regeneration
Myf5
MyoD
Myogenesis
Myogenic progenitor
Paraxial mesoderm
Pax3
Pax7
Pluripotent stem cells
Somite
TGFβ inhibitor
WNT activation
iPS cells

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10.1016/j.semcdb.2017.10.031

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723218

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title = "Myogenic progenitor specification from pluripotent stem cells",

abstract = "Pluripotent stem cells represent important tools for both basic and translational science as they enable to study mechanisms of development, model diseases in vitro and provide a potential source of tissue-specific progenitors for cell therapy. Concomitantly with the increasing knowledge of the molecular mechanisms behind activation of the skeletal myogenic program during embryonic development, novel findings in the stem cell field provided the opportunity to begin recapitulating in vitro the events occurring during specification of the myogenic lineage. In this review, we will provide a perspective of the molecular mechanisms responsible for skeletal myogenic commitment in the embryo and how this knowledge was instrumental for specifying this lineage from pluripotent stem cells. In addition, we will discuss the current limitations for properly recapitulating skeletal myogenesis in the petri dish, and we will provide insights about future applications of pluripotent stem cell-derived myogenic cells.",

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note = "Funding Information: We thank all the investigators in the skeletal muscle and developmental biology fields for their important contributions and, we apologize for those reports that were not included in this review due to space limitations. RCRP is supported by NIH grants R01 AR055299 and R01 AR071439, the Greg Marzolf Jr Foundation, ADVault, Inc and MyDirectives.com. AM thanks the support from Regenerative Medicine Minnesota. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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Myogenic progenitor specification from pluripotent stem cells

Abstract

Bibliographical note

Keywords

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