No evidence for colonization of oral bacteria in the distal gut in healthy adults

Armin Rashidi; Maryam Ebadi; Daniel J. Weisdorf; Massimo Costalonga; Christopher Staley

doi:10.1073/pnas.2114152118

No evidence for colonization of oral bacteria in the distal gut in healthy adults

Armin Rashidi, Maryam Ebadi, Daniel J. Weisdorf, Massimo Costalonga, Christopher Staley

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26 Scopus citations

Abstract

The microbial communities in the mouth and colon are anatomically connected via the saliva. However, the extent to which oral microbes reach and successfully colonize the distal gut has been debated. To resolve this long-standing controversy, we used exact amplicon sequence variants generated from concurrently collected saliva/stool microbiota in 66 healthy adults from two countries to show that, with one exception (Dialister invisus), the two niches are completely distinct. Thus, there is no evidence for colonization of oral bacteria in the distal gut. This defines the healthy state to which pathological states could be compared. Finding the same bacteria in the mouth and stool may warrant clinical investigation for an underlying pathology.

Original language	English (US)
Article number	e2114152118
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	118
Issue number	42
DOIs	https://doi.org/10.1073/pnas.2114152118
State	Published - Oct 19 2021

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. Sequence data were analyzed using the resources of the Minnesota Supercomputing Institute. This work was supported by NIH’s National Center for Advancing Translational Sciences Grant KL2TR002492. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH’s National Center for Advancing Translational Sciences.

Publisher Copyright:
© 2021 National Academy of Sciences. All rights reserved.

Keywords

Dialister invisus
Oral microbiota
gut microbiota

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title = "No evidence for colonization of oral bacteria in the distal gut in healthy adults",

abstract = "The microbial communities in the mouth and colon are anatomically connected via the saliva. However, the extent to which oral microbes reach and successfully colonize the distal gut has been debated. To resolve this long-standing controversy, we used exact amplicon sequence variants generated from concurrently collected saliva/stool microbiota in 66 healthy adults from two countries to show that, with one exception (Dialister invisus), the two niches are completely distinct. Thus, there is no evidence for colonization of oral bacteria in the distal gut. This defines the healthy state to which pathological states could be compared. Finding the same bacteria in the mouth and stool may warrant clinical investigation for an underlying pathology.",

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AU - Ebadi, Maryam

AU - Weisdorf, Daniel J.

AU - Costalonga, Massimo

AU - Staley, Christopher

N1 - Funding Information: ACKNOWLEDGMENTS. Sequence data were analyzed using the resources of the Minnesota Supercomputing Institute. This work was supported by NIH’s National Center for Advancing Translational Sciences Grant KL2TR002492. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH’s National Center for Advancing Translational Sciences. Publisher Copyright: © 2021 National Academy of Sciences. All rights reserved.

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No evidence for colonization of oral bacteria in the distal gut in healthy adults

Abstract

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Keywords

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