Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field 17O MR spectroscopy

Weina Cui; Xiao Hong Zhu; Manda L. Vollmers; Emily T. Colonna; Gregor Adriany; Brandon Tramm; Janet M. Dubinsky; Gülin Öz

doi:10.1038/jcbfm.2013.172

Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field ¹⁷O MR spectroscopy

Weina Cui, Xiao Hong Zhu, Manda L. Vollmers, Emily T. Colonna, Gregor Adriany, Brandon Tramm, Janet M. Dubinsky, Gülin Öz

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. ¹⁷O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO₂) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO₂ measurements with ¹⁷O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.

Original language	English (US)
Pages (from-to)	1846-1849
Number of pages	4
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	33
Issue number	12
DOIs	https://doi.org/10.1038/jcbfm.2013.172
State	Published - Dec 2013

Keywords

Cerebral metabolic rate of oxygen
Magnetic resonance spectroscopic imaging
Mouse
Oxygen-17

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