Abstract
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophilamelanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 853-857 |
| Number of pages | 5 |
| Journal | Nature Methods |
| Volume | 16 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1 2019 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors thank L. Li (X. Zhang Lab at the University of California, Berkeley) for providing gold-evaporated silicon wafer mirrors, S. Köhler (A.F. Dernburg Lab at the University of California, Berkeley) for help with C. elegans samples and A. Bormann and T. Square (C.T. Miller Lab at the University of California, Berkeley) for help with stickleback samples. We thank C.T. Miller, J.W. de Jong and H. Adesnik for discussions. X.Z. acknowledges support from the Gordon and Betty Moore Foundation and the Office of Naval Research Multidisciplinary University Research Initiative program (N00014-17-1-2588). K.X. is a Chan Zuckerberg Biohub investigator and acknowledges support from the Bakar Fellows Award, and STROBE, an NSF Science and Technology Center (DMR 1548924). M.W. acknowledges an NSF Graduate Research Fellowship (DGE-1106400).
Publisher Copyright:
© 2019, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.