Oligomerization of retrovirus integrases

Duane P. Grandgenett; Hideki Aihara

doi:10.1007/978-981-10-8456-0_10

Oligomerization of retrovirus integrases

Duane P. Grandgenett, Hideki Aihara

Chemical and Structural Biology (BMBB)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Integration of the reverse-transcribed viral cDNA into the host’s genome is a critical step in the lifecycle of all retroviruses. Retrovirus integration is carried out by integrase (IN), a virus-encoded enzyme that forms an oligomeric ‘intasome’ complex with both ends of the linear viral DNA to catalyze their concerted insertions into the backbones of the host’s DNA. IN also forms a complex with host proteins, which guides the intasome to the host’s chromosome. Recent structural studies have revealed remarkable diversity as well as conserved features among the architectures of the intasome assembly from different genera of retroviruses. This chapter will review how IN oligomerizes to achieve its function, with particular focus on alpharetrovirus including the avian retrovirus Rous sarcoma virus. Another chapter (Craigie) will focus on the structure and function of IN from HIV-1.

Original language	English (US)
Title of host publication	Subcellular Biochemistry
Publisher	Springer New York
Pages	211-243
Number of pages	33
DOIs	https://doi.org/10.1007/978-981-10-8456-0_10
State	Published - 2018

Publication series

Name	Subcellular Biochemistry
Volume	88
ISSN (Print)	0306-0225

Bibliographical note

Publisher Copyright:
© Springer Nature Singapore Pte Ltd. 2018.

Keywords

Alpharetrovirus
Avian sarcoma/leukemia (ASLV)
Betaretrovirus
Bromodomain and extra-terminal (BET)
C-terminal domain (CTD)
Catalytic core domain (CCD)
Concerted integration
Conserved intasome core (CIC)
Cryo electron microscopy (EM)
Crystal structure
D,D(35)E motif
FAcilitates Chromatin Transcription (FACT)
Human immunodeficiency virus type 1 (HIV-1)
Intasome
Integrase (IN)
Lens epithelium-derived growth factor (LEDGF/p75)
Lentivirus
Mouse mammary tumor virus (MMTV)
N-terminal domain (NTD)
Preintegration complex (PIC)
Prototype foamy virus (PFV)
Retrovirus
Rous sarcoma virus (RSV)
Spumavirus
Stable synaptic complex (SSC)
Strand transfer complex (STC)
Strand transfer inhibitor (STI)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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AB - Integration of the reverse-transcribed viral cDNA into the host’s genome is a critical step in the lifecycle of all retroviruses. Retrovirus integration is carried out by integrase (IN), a virus-encoded enzyme that forms an oligomeric ‘intasome’ complex with both ends of the linear viral DNA to catalyze their concerted insertions into the backbones of the host’s DNA. IN also forms a complex with host proteins, which guides the intasome to the host’s chromosome. Recent structural studies have revealed remarkable diversity as well as conserved features among the architectures of the intasome assembly from different genera of retroviruses. This chapter will review how IN oligomerizes to achieve its function, with particular focus on alpharetrovirus including the avian retrovirus Rous sarcoma virus. Another chapter (Craigie) will focus on the structure and function of IN from HIV-1.

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Oligomerization of retrovirus integrases

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

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