On‐line monitoring of hybridoma cell growth using a laser turbidity sensor

Konstantin B. Konstantinov; Rindit Pambayun; Ricaredo Matanguihan; Toshiomi Yoshida; Cheryl M. Perusicn; Wei‐Shou ‐S Hu

doi:10.1002/bit.260401107

On‐line monitoring of hybridoma cell growth using a laser turbidity sensor

Konstantin B. Konstantinov, Rindit Pambayun, Ricaredo Matanguihan, Toshiomi Yoshida, Cheryl M. Perusicn, Wei‐Shou ‐S Hu

Chemical Engineering and Materials Science

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Abstract

A high‐sensitivity turbidity probe was used for on‐line monitoring of the cell concentration in batch hybridoma cultivation. Good correlation between off‐line cell counts and the linearized sensor signal was found. The quality of the signal was sufficiently high to provide for on‐line estimation of the specific growth rate using an efficient filtering procedure. These positive results suggest that such laser turbidity sensors will facilitate development of systems for on‐line monitoring and control of animal cell cultivations. © 1992 John Wiley & Sons, Inc.

Original language	English (US)
Pages (from-to)	1337-1342
Number of pages	6
Journal	Biotechnology and bioengineering
Volume	40
Issue number	11
DOIs	https://doi.org/10.1002/bit.260401107
State	Published - Dec 20 1992

Keywords

bioprocess control
cell concentration monitoring
growth rate
hybridoma growth
turbidity sensor

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title = "On‐line monitoring of hybridoma cell growth using a laser turbidity sensor",

abstract = "A high‐sensitivity turbidity probe was used for on‐line monitoring of the cell concentration in batch hybridoma cultivation. Good correlation between off‐line cell counts and the linearized sensor signal was found. The quality of the signal was sufficiently high to provide for on‐line estimation of the specific growth rate using an efficient filtering procedure. These positive results suggest that such laser turbidity sensors will facilitate development of systems for on‐line monitoring and control of animal cell cultivations. {\textcopyright} 1992 John Wiley & Sons, Inc.",

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AU - Matanguihan, Ricaredo

AU - Yoshida, Toshiomi

AU - Perusicn, Cheryl M.

AU - Hu, Wei‐Shou ‐S

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KW - cell concentration monitoring

KW - growth rate

KW - hybridoma growth

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On‐line monitoring of hybridoma cell growth using a laser turbidity sensor

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