Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep

Christa J. Van Dort; Daniel P. Zachs; Jonathan D. Kenny; Shu Zheng; Rebecca R. Goldblum; Noah A. Gelwan; Daniel M. Ramos; Michael A. Nolan; Karen Wang; Feng Ju Weng; Yingxi Lin; Matthew A. Wilson; Emery N. Brown

doi:10.1073/pnas.1423136112

Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep

Christa J. Van Dort, Daniel P. Zachs, Jonathan D. Kenny, Shu Zheng, Rebecca R. Goldblum, Noah A. Gelwan, Daniel M. Ramos, Michael A. Nolan, Karen Wang, Feng Ju Weng, Yingxi Lin, Matthew A. Wilson, Emery N. Brown

Research output: Contribution to journal › Article › peer-review

210 Scopus citations

Abstract

Rapid eye movement (REM) sleep is an important component of the natural sleep/wake cycle, yet the mechanisms that regulate REM sleep remain incompletely understood. Cholinergic neurons in the mesopontine tegmentum have been implicated in REM sleep regulation, but lesions of this area have had varying effects on REM sleep. Therefore, this study aimed to clarify the role of cholinergic neurons in the pedunculopontine tegmentum (PPT) and laterodorsal tegmentum (LDT) in REM sleep generation. Selective optogenetic activation of cholinergic neurons in the PPT or LDT during non-REM (NREM) sleep increased the number of REM sleep episodes and did not change REM sleep episode duration. Activation of cholinergic neurons in the PPT or LDT during NREM sleep was sufficient to induce REM sleep.

Original language	English (US)
Pages (from-to)	584-589
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	112
Issue number	2
DOIs	https://doi.org/10.1073/pnas.1423136112
State	Published - Jan 13 2015
Externally published	Yes

Keywords

Acetylcholine
Mesopontine tegmentum
Mouse
Optogenetics
Rapid eye movement sleep

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Van Dort, C. J., Zachs, D. P., Kenny, J. D., Zheng, S., Goldblum, R. R., Gelwan, N. A., Ramos, D. M., Nolan, M. A., Wang, K., Weng, F. J., Lin, Y., Wilson, M. A., & Brown, E. N. (2015). Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep. Proceedings of the National Academy of Sciences of the United States of America, 112(2), 584-589. https://doi.org/10.1073/pnas.1423136112

Van Dort, CJ, Zachs, DP, Kenny, JD, Zheng, S, Goldblum, RR, Gelwan, NA, Ramos, DM, Nolan, MA, Wang, K, Weng, FJ, Lin, Y, Wilson, MA & Brown, EN 2015, 'Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep', Proceedings of the National Academy of Sciences of the United States of America, vol. 112, no. 2, pp. 584-589. https://doi.org/10.1073/pnas.1423136112

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abstract = "Rapid eye movement (REM) sleep is an important component of the natural sleep/wake cycle, yet the mechanisms that regulate REM sleep remain incompletely understood. Cholinergic neurons in the mesopontine tegmentum have been implicated in REM sleep regulation, but lesions of this area have had varying effects on REM sleep. Therefore, this study aimed to clarify the role of cholinergic neurons in the pedunculopontine tegmentum (PPT) and laterodorsal tegmentum (LDT) in REM sleep generation. Selective optogenetic activation of cholinergic neurons in the PPT or LDT during non-REM (NREM) sleep increased the number of REM sleep episodes and did not change REM sleep episode duration. Activation of cholinergic neurons in the PPT or LDT during NREM sleep was sufficient to induce REM sleep.",

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AU - Van Dort, Christa J.

AU - Zachs, Daniel P.

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AU - Goldblum, Rebecca R.

AU - Gelwan, Noah A.

AU - Ramos, Daniel M.

AU - Nolan, Michael A.

AU - Wang, Karen

AU - Weng, Feng Ju

AU - Lin, Yingxi

AU - Wilson, Matthew A.

AU - Brown, Emery N.

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Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep

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