TY - JOUR
T1 - Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in Drosophila photoreceptors
AU - Gonzalez-Bellido, Paloma T.
AU - Wardill, Trevor J.
AU - Kostyleva, Ripsik
AU - Meinertzhagen, Ian A.
AU - Juusola, Mikko
PY - 2009/11/11
Y1 - 2009/11/11
N2 - shibirets1, a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibirets1 has been used to dissect neuronal circuits. We investigated the effects of UAS-shibirets1 overexpression in Drosophila photoreceptors at permissive (19° C) and restrictive (31° C) temperatures. At 19° C, overexpression of UAS-shits1 causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibire ts1 contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-electron microscopy reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibirets1 overexpression in lamina cells also bundles microtubules.Weconclude that dynamin has multiple functions that are interrupted by UAS-shibirets1 overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.
AB - shibirets1, a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibirets1 has been used to dissect neuronal circuits. We investigated the effects of UAS-shibirets1 overexpression in Drosophila photoreceptors at permissive (19° C) and restrictive (31° C) temperatures. At 19° C, overexpression of UAS-shits1 causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibire ts1 contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-electron microscopy reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibirets1 overexpression in lamina cells also bundles microtubules.Weconclude that dynamin has multiple functions that are interrupted by UAS-shibirets1 overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.
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U2 - 10.1523/JNEUROSCI.2873-09.2009
DO - 10.1523/JNEUROSCI.2873-09.2009
M3 - Article
C2 - 19906968
AN - SCOPUS:70449629771
SN - 0270-6474
VL - 29
SP - 14199
EP - 14210
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 45
ER -