Performance of a High-Pressure Liquid Chromatography-Ion Mobility-Mass Spectrometry System for Metabolic Profiling

Xing Zhang; Kimberly Kew; Richard Reisdorph; Mark Sartain; Roger Powell; Michael Armstrong; Kevin Quinn; Charmion Cruickshank-Quinn; Scott Walmsley; Samantha Bokatzian; Ed Darland; Matthew Rain; Ken Imatani; Nichole Reisdorph

doi:10.1021/acs.analchem.6b04628

Performance of a High-Pressure Liquid Chromatography-Ion Mobility-Mass Spectrometry System for Metabolic Profiling

Xing Zhang, Kimberly Kew, Richard Reisdorph, Mark Sartain, Roger Powell, Michael Armstrong, Kevin Quinn, Charmion Cruickshank-Quinn, Scott Walmsley, Samantha Bokatzian, Ed Darland, Matthew Rain, Ken Imatani, Nichole Reisdorph

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A commercial liquid chromatography/drift tube ion mobility-mass spectrometer (LC/IM-MS) was evaluated for its utility in global metabolomics analysis. Performance was assessed using 12 targeted metabolite standards where the limit of detection (LOD), linear dynamic range, resolving power, and collision cross section (Ω) are reported for each standard. Data were collected in three different instrument operation modes: flow injection analysis with IM-MS (FIA/IM-MS), LC/MS, and LC/IM-MS. Metabolomics analyses of human plasma and HaCaT cells were used to compare the above three operation modes. LC/MS provides linearity in response, data processing automation, improved limits of detection, and ease of use. Advantages of LC/IM-MS and FIA/IM-MS include the ability to develop mobility-mass trend lines for structurally similar biomolecules, increased peak capacity, reduction of chemical/matrix noise, improvement in signal-to-noise, and separations of isobar/isomer compounds that are not resolved by LC. We further tested the feasibility of incorporating IM-MS into conventional LC/MS metabolomics workflows. In general, the addition of ion mobility dimension has increased the separation of compounds in complex biological matrixes and has the potential to largely improve the throughput of metabolomics analysis.

Original language	English (US)
Pages (from-to)	6384-6391
Number of pages	8
Journal	Analytical Chemistry
Volume	89
Issue number	12
DOIs	https://doi.org/10.1021/acs.analchem.6b04628
State	Published - Jun 20 2017
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the DARPA award 13-34-RTA-FP- 007 to William Old (N. Reisdorph, subcontractor). The opinions, points of view, findings, and conclusions or recommendations expressed in this publication represent a consensus of the authors and do not necessarily represent the official position or policies of the U.S. Department of Defense. Any products and manufacturers discussed are presented for informational purposes only and do not constitute product approval or endorsement by the U.S. Department of Defense.

Publisher Copyright:
© 2017 American Chemical Society.

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Zhang, X., Kew, K., Reisdorph, R., Sartain, M., Powell, R., Armstrong, M., Quinn, K., Cruickshank-Quinn, C., Walmsley, S., Bokatzian, S., Darland, E., Rain, M., Imatani, K., & Reisdorph, N. (2017). Performance of a High-Pressure Liquid Chromatography-Ion Mobility-Mass Spectrometry System for Metabolic Profiling. Analytical Chemistry, 89(12), 6384-6391. https://doi.org/10.1021/acs.analchem.6b04628

Zhang, X, Kew, K, Reisdorph, R, Sartain, M, Powell, R, Armstrong, M, Quinn, K, Cruickshank-Quinn, C, Walmsley, S, Bokatzian, S, Darland, E, Rain, M, Imatani, K & Reisdorph, N 2017, 'Performance of a High-Pressure Liquid Chromatography-Ion Mobility-Mass Spectrometry System for Metabolic Profiling', Analytical Chemistry, vol. 89, no. 12, pp. 6384-6391. https://doi.org/10.1021/acs.analchem.6b04628

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abstract = "A commercial liquid chromatography/drift tube ion mobility-mass spectrometer (LC/IM-MS) was evaluated for its utility in global metabolomics analysis. Performance was assessed using 12 targeted metabolite standards where the limit of detection (LOD), linear dynamic range, resolving power, and collision cross section (Ω) are reported for each standard. Data were collected in three different instrument operation modes: flow injection analysis with IM-MS (FIA/IM-MS), LC/MS, and LC/IM-MS. Metabolomics analyses of human plasma and HaCaT cells were used to compare the above three operation modes. LC/MS provides linearity in response, data processing automation, improved limits of detection, and ease of use. Advantages of LC/IM-MS and FIA/IM-MS include the ability to develop mobility-mass trend lines for structurally similar biomolecules, increased peak capacity, reduction of chemical/matrix noise, improvement in signal-to-noise, and separations of isobar/isomer compounds that are not resolved by LC. We further tested the feasibility of incorporating IM-MS into conventional LC/MS metabolomics workflows. In general, the addition of ion mobility dimension has increased the separation of compounds in complex biological matrixes and has the potential to largely improve the throughput of metabolomics analysis.",

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AU - Armstrong, Michael

AU - Quinn, Kevin

AU - Cruickshank-Quinn, Charmion

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AU - Bokatzian, Samantha

AU - Darland, Ed

AU - Rain, Matthew

AU - Imatani, Ken

AU - Reisdorph, Nichole

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Performance of a High-Pressure Liquid Chromatography-Ion Mobility-Mass Spectrometry System for Metabolic Profiling

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