TY - JOUR
T1 - Phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia involves CD14 receptors
AU - Lipovsky, Myriam M.
AU - Gekker, Genya
AU - Anderson, W. Robert
AU - Molitor, Thomas W.
AU - Peterson, Phillip K.
AU - Hoepelman, Andy I.M.
PY - 1997/8
Y1 - 1997/8
N2 - The interaction of the opportunistic fungus Cryptococcus neoformans with swine microglia was studied in vitro in the presence and absence of anti- CD14 monoclonal antibodies. In the absence of anti-CD14 antibodies, 36% of microglia had phagocytized nonopsonized, encapsulated cryptococci after 2 hr incubation (effector-to-target ratio, 1:50). Preincubation of microglia with anti-CD14 antibodies resulted in a 63% reduction of phagocytosis. These findings suggest that CD14 receptors facilitate uptake of nonopsonized C. neoformans by resident macrophages within the brain.
AB - The interaction of the opportunistic fungus Cryptococcus neoformans with swine microglia was studied in vitro in the presence and absence of anti- CD14 monoclonal antibodies. In the absence of anti-CD14 antibodies, 36% of microglia had phagocytized nonopsonized, encapsulated cryptococci after 2 hr incubation (effector-to-target ratio, 1:50). Preincubation of microglia with anti-CD14 antibodies resulted in a 63% reduction of phagocytosis. These findings suggest that CD14 receptors facilitate uptake of nonopsonized C. neoformans by resident macrophages within the brain.
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U2 - 10.1006/clin.1997.4381
DO - 10.1006/clin.1997.4381
M3 - Article
C2 - 9245554
AN - SCOPUS:0031214280
SN - 0090-1229
VL - 84
SP - 208
EP - 211
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -