TY - JOUR
T1 - Physical mapping of DNA markers linked to stem rust resistance gene Sr47 in durum wheat
AU - Klindworth, Daryl L.
AU - Saini, Jyoti
AU - Long, Yunming
AU - Rouse, Matthew N.
AU - Faris, Justin D.
AU - Jin, Yue
AU - Xu, Steven S.
N1 - Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg (outside the USA).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Key message: Markers linked to stem rust resistance geneSr47were physically mapped in three smallAegilops speltoideschromosomal bins. Five markers, including two PCR-based SNP markers, were validated for marker-assisted selection. Abstract: In durum wheat (Triticum turgidum subsp. durum), the gene Sr47 derived from Aegilopsspeltoides conditions resistance to race TTKSK (Ug99) of the stem rust pathogen (Puccinia graminis f. sp. tritici). Sr47 is carried on small interstitial translocation chromosomes (Ti2BL-2SL-2BL·2BS) in which the Ae. speltoides chromosome 2S segments are divided into four bins in genetic stocks RWG35, RWG36, and RWG37. Our objective was to physically map molecular markers to bins and to determine if any of the molecular markers would be useful in marker-assisted selection (MAS). Durum cultivar Joppa was used as the recurrent parent to produce three BC2F2 populations. Each BC2F2 plant was genotyped with markers to detect the segment carrying Sr47, and stem rust testing of BC2F3 progeny with race TTKSK confirmed the genotyping. Forty-nine markers from published sources, four new SSR markers, and five new STARP (semi-thermal asymmetric reverse PCR) markers, were evaluated in BC2F2 populations for assignment of markers to bins. Sr47 was mapped to bin 3 along with 13 markers. No markers were assigned to bin 1; however, 7 and 13 markers were assigned to bins 2 and 4, respectively. Markers Xrwgs38a, Xmag1729, Xwmc41, Xtnac3119, Xrwgsnp1, and Xrwgsnp4 were found to be useful for MAS of Sr47. However, STARP markers Xrwgsnp1 and Xrwgsnp4 can be used in gel-free systems, and are the preferred markers for high-throughput MAS. The physical mapping data from this study will also be useful for pyramiding Sr47 with other Sr genes on chromosome 2B.
AB - Key message: Markers linked to stem rust resistance geneSr47were physically mapped in three smallAegilops speltoideschromosomal bins. Five markers, including two PCR-based SNP markers, were validated for marker-assisted selection. Abstract: In durum wheat (Triticum turgidum subsp. durum), the gene Sr47 derived from Aegilopsspeltoides conditions resistance to race TTKSK (Ug99) of the stem rust pathogen (Puccinia graminis f. sp. tritici). Sr47 is carried on small interstitial translocation chromosomes (Ti2BL-2SL-2BL·2BS) in which the Ae. speltoides chromosome 2S segments are divided into four bins in genetic stocks RWG35, RWG36, and RWG37. Our objective was to physically map molecular markers to bins and to determine if any of the molecular markers would be useful in marker-assisted selection (MAS). Durum cultivar Joppa was used as the recurrent parent to produce three BC2F2 populations. Each BC2F2 plant was genotyped with markers to detect the segment carrying Sr47, and stem rust testing of BC2F3 progeny with race TTKSK confirmed the genotyping. Forty-nine markers from published sources, four new SSR markers, and five new STARP (semi-thermal asymmetric reverse PCR) markers, were evaluated in BC2F2 populations for assignment of markers to bins. Sr47 was mapped to bin 3 along with 13 markers. No markers were assigned to bin 1; however, 7 and 13 markers were assigned to bins 2 and 4, respectively. Markers Xrwgs38a, Xmag1729, Xwmc41, Xtnac3119, Xrwgsnp1, and Xrwgsnp4 were found to be useful for MAS of Sr47. However, STARP markers Xrwgsnp1 and Xrwgsnp4 can be used in gel-free systems, and are the preferred markers for high-throughput MAS. The physical mapping data from this study will also be useful for pyramiding Sr47 with other Sr genes on chromosome 2B.
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U2 - 10.1007/s00122-017-2875-7
DO - 10.1007/s00122-017-2875-7
M3 - Article
C2 - 28286900
AN - SCOPUS:85014925609
SN - 0040-5752
VL - 130
SP - 1135
EP - 1154
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 6
ER -