TY - JOUR
T1 - Plasma-induced inactivation of Staphylococcus aureus biofilms
T2 - The role of atomic oxygen and comparison with disinfectants and antibiotics
AU - Nandula, Seshagiri R.
AU - Kondeti, Vighneswara S.S.K.
AU - Phan, Chi
AU - Wang, Jianan
AU - Penningroth, Mitchell R.
AU - Granick, Jennifer L.
AU - Bruggeman, Peter J.
AU - Hunter, Ryan C.
N1 - Publisher Copyright:
© 2022 The Authors. Plasma Processes and Polymers published by Wiley-VCH GmbH.
PY - 2023/1
Y1 - 2023/1
N2 - Microbial biofilms are of critical concern because of their recalcitrance to antimicrobials. Cold atmospheric plasmas (CAP) represent a promising biofilm remediation strategy as they generate reactive oxygen and nitrogen species (RONS), but mechanisms underpinning CAP-biofilm interactions remain unknown. We assess the impact of treatment modality on biofilm inactivation and show that CAP killing of Staphylococcus aureus biofilms is dependent on treatment conditions, including solution chemistry. In dry treatments, biofilms are locally ablated due to plasma-produced O flux. For saline-submerged biofilms, while we show that ClO− is generated at high concentrations in larger treatment volumes, CAP inactivation at low ClO− concentrations implicates other reaction pathways. Finally, we demonstrate CAP efficacy over conventional antimicrobials, underscoring its promise as a biofilm treatment approach.
AB - Microbial biofilms are of critical concern because of their recalcitrance to antimicrobials. Cold atmospheric plasmas (CAP) represent a promising biofilm remediation strategy as they generate reactive oxygen and nitrogen species (RONS), but mechanisms underpinning CAP-biofilm interactions remain unknown. We assess the impact of treatment modality on biofilm inactivation and show that CAP killing of Staphylococcus aureus biofilms is dependent on treatment conditions, including solution chemistry. In dry treatments, biofilms are locally ablated due to plasma-produced O flux. For saline-submerged biofilms, while we show that ClO− is generated at high concentrations in larger treatment volumes, CAP inactivation at low ClO− concentrations implicates other reaction pathways. Finally, we demonstrate CAP efficacy over conventional antimicrobials, underscoring its promise as a biofilm treatment approach.
KW - Staphylococcus aureus
KW - biofilm
KW - cold atmospheric plasma
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U2 - 10.1002/ppap.202200147
DO - 10.1002/ppap.202200147
M3 - Article
AN - SCOPUS:85139676047
SN - 1612-8850
VL - 20
JO - Plasma Processes and Polymers
JF - Plasma Processes and Polymers
IS - 1
M1 - 2200147
ER -