Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin

Manuel Toledano-Osorio; Raquel Osorio; Fátima S. Aguilera; Antonio Luis Medina-Castillo; Manuel Toledano; Estrella Osorio; Sergio Acosta; Ruoqiong Chen; Conrado Aparicio

doi:10.1016/j.actbio.2020.05.002

Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin

Manuel Toledano-Osorio, Raquel Osorio, Fátima S. Aguilera, Antonio Luis Medina-Castillo, Manuel Toledano, Estrella Osorio, Sergio Acosta, Ruoqiong Chen, Conrado Aparicio

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. Statement of significance: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface.

Original language	English (US)
Pages (from-to)	316-326
Number of pages	11
Journal	Acta Biomaterialia
Volume	111
DOIs	https://doi.org/10.1016/j.actbio.2020.05.002
State	Published - Jul 15 2020

Bibliographical note

Funding Information:
This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers ( http://uic.umn.edu) .

Funding Information:
This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers (http://uic.umn.edu). The authors report no conflicts of interest in this work.

Publisher Copyright:
© 2020

Keywords

Biofilm
Degradation
Dentin
Interface
Nanoparticle

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10.1016/j.actbio.2020.05.002

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Toledano-Osorio, M., Osorio, R., Aguilera, F. S., Medina-Castillo, A. L., Toledano, M., Osorio, E., Acosta, S., Chen, R., & Aparicio, C. (2020). Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin. Acta Biomaterialia, 111, 316-326. https://doi.org/10.1016/j.actbio.2020.05.002

Toledano-Osorio, M, Osorio, R, Aguilera, FS, Medina-Castillo, AL, Toledano, M, Osorio, E, Acosta, S, Chen, R & Aparicio, C 2020, 'Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin', Acta Biomaterialia, vol. 111, pp. 316-326. https://doi.org/10.1016/j.actbio.2020.05.002

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title = "Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin",

abstract = "The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD{\textregistered} viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. Statement of significance: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface.",

keywords = "Biofilm, Degradation, Dentin, Interface, Nanoparticle",

author = "Manuel Toledano-Osorio and Raquel Osorio and Aguilera, {F{\'a}tima S.} and Medina-Castillo, {Antonio Luis} and Manuel Toledano and Estrella Osorio and Sergio Acosta and Ruoqiong Chen and Conrado Aparicio",

note = "Funding Information: This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers ( http://uic.umn.edu) . Funding Information: This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers (http://uic.umn.edu). The authors report no conflicts of interest in this work. Publisher Copyright: {\textcopyright} 2020",

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T2 - Nanoparticles antidegradation activity at bonded dentin

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AU - Aguilera, Fátima S.

AU - Medina-Castillo, Antonio Luis

AU - Toledano, Manuel

AU - Osorio, Estrella

AU - Acosta, Sergio

AU - Chen, Ruoqiong

AU - Aparicio, Conrado

N1 - Funding Information: This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers ( http://uic.umn.edu) . Funding Information: This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers (http://uic.umn.edu). The authors report no conflicts of interest in this work. Publisher Copyright: © 2020

PY - 2020/7/15

Y1 - 2020/7/15

N2 - The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. Statement of significance: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface.

AB - The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. Statement of significance: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface.

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KW - Nanoparticle

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Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin

Abstract

Bibliographical note

Keywords

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Light Microscopy

Nikon A1RHD Multiphoton Upright Confocal

University Imaging Centers

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Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Equipment

Light Microscopy

Nikon A1RHD Multiphoton Upright Confocal

University Imaging Centers

Cite this