TY - JOUR
T1 - Porcine myelomonocytic markers
T2 - Summary of the Second International Swine CD Workshop
AU - Domínguez, J.
AU - Ezquerra, A.
AU - Alonso, F.
AU - McCullough, K.
AU - Summerfield, A.
AU - Bianchi, A.
AU - Zwart, R. J.
AU - Kim, Y. B.
AU - Blecha, F.
AU - Eicher, S.
AU - Murtaugh, M.
AU - Pampusch, M.
AU - Burger, K.
PY - 1998/1/30
Y1 - 1998/1/30
N2 - Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic ceils was established in this workshop.
AB - Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic ceils was established in this workshop.
KW - CD14 mAbs
KW - Flow cytometry
KW - Immunohistochemistry
KW - Porcine myelomonocytic markers
UR - http://www.scopus.com/inward/record.url?scp=17344378904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17344378904&partnerID=8YFLogxK
U2 - 10.1016/S0165-2427(97)00109-8
DO - 10.1016/S0165-2427(97)00109-8
M3 - Article
C2 - 9589571
AN - SCOPUS:17344378904
SN - 0165-2427
VL - 60
SP - 329
EP - 341
JO - Veterinary immunology and immunopathology
JF - Veterinary immunology and immunopathology
IS - 3-4
ER -