Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles

Boris V. Syomin; Konstantin V. Kandror; Andrej B. Semakin; Vladimir L. Tsuprun; Alexander S. Stepanov

doi:10.1016/0014-5793(93)81358-7

Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles

Boris V. Syomin, Konstantin V. Kandror, Andrej B. Semakin, Vladimir L. Tsuprun, Alexander S. Stepanov

Otolaryngology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA⁺ RNA and polyA⁺ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.

Original language	English (US)
Pages (from-to)	285-288
Number of pages	4
Journal	FEBS Letters
Volume	323
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)81358-7
State	Published - Jun 1 1993

Bibliographical note

Funding Information:
Ash-no,~ledgrnzWeerz thã,nsk. Yurn Ilyin and Nataha Lyubomirskaya for helpful comments on the manuscript. This work was supported by the Russian State Program ‘Frontiers m genetrcs’.

Keywords

Retrotransposon
Virus-like particle
gypsy

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title = "Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles",

abstract = "As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.",

keywords = "Retrotransposon, Virus-like particle, gypsy",

author = "Syomin, {Boris V.} and Kandror, {Konstantin V.} and Semakin, {Andrej B.} and Tsuprun, {Vladimir L.} and Stepanov, {Alexander S.}",

note = "Funding Information: Ash-no,~ledgrnzWeerz th{\~a},nsk. Yurn Ilyin and Nataha Lyubomirskaya for helpful comments on the manuscript. This work was supported by the Russian State Program {\textquoteleft}Frontiers m genetrcs{\textquoteright}.",

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AU - Syomin, Boris V.

AU - Kandror, Konstantin V.

AU - Semakin, Andrej B.

AU - Tsuprun, Vladimir L.

AU - Stepanov, Alexander S.

N1 - Funding Information: Ash-no,~ledgrnzWeerz thã,nsk. Yurn Ilyin and Nataha Lyubomirskaya for helpful comments on the manuscript. This work was supported by the Russian State Program ‘Frontiers m genetrcs’.

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N2 - As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.

AB - As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.

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Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles

Abstract

Bibliographical note

Keywords

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