Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

Jiang Li; Chen Tan; Qiu Xiang; Xiaomei Zhang; Jian Ma; Jie ru Wang; Jianbo Yang; Wei fang Li; Shou rong Shen; Songping Liang; Guiyuan Li

doi:10.1023/A:1010912311564

Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

Jiang Li, Chen Tan, Qiu Xiang, Xiaomei Zhang, Jian Ma, Jie ru Wang, Jianbo Yang, Wei fang Li, Shou rong Shen, Songping Liang, Guiyuan Li

Laboratory Medicine and Pathology

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Abstract

In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.

Original language	English (US)
Pages (from-to)	265-271
Number of pages	7
Journal	Journal of Protein Chemistry
Volume	20
Issue number	3
DOIs	https://doi.org/10.1023/A:1010912311564
State	Published - 2001

Keywords

Mass spectrometry
Protein identification
Two-dimensional gel electrophoresis

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title = "Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells",

abstract = "In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.",

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T1 - Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

AU - Li, Jiang

AU - Tan, Chen

AU - Xiang, Qiu

AU - Zhang, Xiaomei

AU - Ma, Jian

AU - Wang, Jie ru

AU - Yang, Jianbo

AU - Li, Wei fang

AU - Shen, Shou rong

AU - Liang, Songping

AU - Li, Guiyuan

PY - 2001

Y1 - 2001

N2 - In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.

AB - In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.

KW - Mass spectrometry

KW - Protein identification

KW - Two-dimensional gel electrophoresis

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Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

Abstract

Keywords

UN SDGs

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