Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration

Nelson J. Rodríguez-Merced; Mackenzie K. Callaway; Paolo P. Provenzano

doi:10.1016/j.xpro.2023.102135

Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration

Nelson J. Rodríguez-Merced, Mackenzie K. Callaway, Paolo P. Provenzano

Biomedical Engineering

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Abstract

Here, we describe a protocol for culture and live cell imaging of tumor slices. This approach studies carcinoma and immune cell dynamics in complex tumor microenvironments (TME) with nonlinear optical imaging platforms. Using a tumor-bearing mouse model of pancreatic ductal adenocarcinoma (PDA), we detail steps to isolate, activate, and label CD8⁺ T lymphocytes and later introduce them to live murine PDA tumor slice explants. The techniques described in this protocol can improve our understanding of cell migration in complex microenvironments ex vivo. For complete details on the use and execution of this protocol, please refer to Tabdanov et al. (2021).¹

Original language	English (US)
Article number	102135
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2023.102135
State	Published - Mar 17 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Keywords

Cancer
Cell Biology
Immunology
Microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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KW - Cell Biology

KW - Immunology

KW - Microscopy

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Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration

Abstract

Bibliographical note

Keywords

UN SDGs

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