TY - JOUR
T1 - Purification to apparent homogeneity of a μ-type opioid receptor from rat brain
AU - Cho, T. M.
AU - Hasegawa, J. I.
AU - Ge, B. L.
AU - Loh, H. H.
PY - 1986
Y1 - 1986
N2 - A μ-opioid-specific receptor was purified to apparent homogeneity from rat brain membranes by 6-succinylmorphine affinity chromatography, gel filtration, wheat germ agglutinin affinity chromatography, and isoelectric focusing. The purified receptor had a molecular weight of 58,000 as determined by NaDodSO4/polyacrylamide gel electrophoresis and was judged to be homogeneous by the following criteria: (i) a single band was detected by autoradiography after NaDodSO4/polyacrylamide gel electrophoresis of 125I-labeled receptor and (ii) the purified preparation had a specific opioid-binding activity of 17,720 pmol/mg of protein, close to the theoretical value. In addition, the M(r) 58,000 value agrees closely with that determined by covalently labeling purified receptor with bromoacetyl-[3H]dihydromorphine or with125I-labeled β-endorphin and dimethyl suberimidate.
AB - A μ-opioid-specific receptor was purified to apparent homogeneity from rat brain membranes by 6-succinylmorphine affinity chromatography, gel filtration, wheat germ agglutinin affinity chromatography, and isoelectric focusing. The purified receptor had a molecular weight of 58,000 as determined by NaDodSO4/polyacrylamide gel electrophoresis and was judged to be homogeneous by the following criteria: (i) a single band was detected by autoradiography after NaDodSO4/polyacrylamide gel electrophoresis of 125I-labeled receptor and (ii) the purified preparation had a specific opioid-binding activity of 17,720 pmol/mg of protein, close to the theoretical value. In addition, the M(r) 58,000 value agrees closely with that determined by covalently labeling purified receptor with bromoacetyl-[3H]dihydromorphine or with125I-labeled β-endorphin and dimethyl suberimidate.
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U2 - 10.1073/pnas.83.12.4138
DO - 10.1073/pnas.83.12.4138
M3 - Article
C2 - 3012554
AN - SCOPUS:0022536216
SN - 0027-8424
VL - 83
SP - 4138
EP - 4142
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -