Quantitative and real-time detection of secretion of chemical messengers from individual platelets

Shencheng Ge; Nathan J. Wittenberg; Christy L. Haynes

doi:10.1021/bi800792m

Quantitative and real-time detection of secretion of chemical messengers from individual platelets

Shencheng Ge, Nathan J. Wittenberg, Christy L. Haynes

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a δ-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the δ-granules.

Original language	English (US)
Pages (from-to)	7020-7024
Number of pages	5
Journal	Biochemistry
Volume	47
Issue number	27
DOIs	https://doi.org/10.1021/bi800792m
State	Published - Jul 8 2008

Access

10.1021/bi800792m

OpenUrl availability

Full text

Cite this

@article{f4cbcffb3f554b33a21902468ab6239b,

title = "Quantitative and real-time detection of secretion of chemical messengers from individual platelets",

abstract = "Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a δ-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the δ-granules.",

author = "Shencheng Ge and Wittenberg, {Nathan J.} and Haynes, {Christy L.}",

year = "2008",

month = jul,

day = "8",

doi = "10.1021/bi800792m",

language = "English (US)",

volume = "47",

pages = "7020--7024",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

number = "27",

}

TY - JOUR

T1 - Quantitative and real-time detection of secretion of chemical messengers from individual platelets

AU - Ge, Shencheng

AU - Wittenberg, Nathan J.

AU - Haynes, Christy L.

PY - 2008/7/8

Y1 - 2008/7/8

N2 - Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a δ-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the δ-granules.

AB - Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a δ-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the δ-granules.

UR - http://www.scopus.com/inward/record.url?scp=46849106473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46849106473&partnerID=8YFLogxK

U2 - 10.1021/bi800792m

DO - 10.1021/bi800792m

M3 - Article

C2 - 18557631

AN - SCOPUS:46849106473

SN - 0006-2960

VL - 47

SP - 7020

EP - 7024

JO - Biochemistry

JF - Biochemistry

IS - 27

ER -

Quantitative and real-time detection of secretion of chemical messengers from individual platelets

Abstract

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this