TY - JOUR
T1 - Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media
AU - Monsanto, Rafael da Costa
AU - Penido, Norma de Oliveira
AU - Uchiyama, Mio
AU - Schachern, Patricia
AU - Paparella, Michael M.
AU - Cureoglu, Sebahattin
N1 - Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2021/2
Y1 - 2021/2
N2 - Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.
AB - Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.
KW - Chronic otitis media
KW - Hearing loss
KW - Otitis media
KW - Scarpa ganglion cells
KW - Spiral ganglion cells
KW - Tinnitus
KW - Vestibular diseases
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U2 - 10.1007/s00405-020-06094-5
DO - 10.1007/s00405-020-06094-5
M3 - Article
C2 - 32488375
AN - SCOPUS:85085869658
SN - 0937-4477
VL - 278
SP - 331
EP - 338
JO - European Archives of Oto-Rhino-Laryngology
JF - European Archives of Oto-Rhino-Laryngology
IS - 2
ER -