Abstract
Aim, materials & methods: Urinary cortisol profile has the potential as a diagnostic biomarker. We therefore developed a stable-isotope dilution ultraperformance chromatography multistage MS-based method to quantify cortisol and 16 metabolites in human urines. Results & conclusion: The LOD for cortisol and its metabolites ranges from 0.02 to 5.81 pg/μl urine. The inter- and intraday variations were 3.7-12.9% and 3.5-15.6%, respectively. Among the metabolites analyzed, significant person-to-person heterogeneity was observed, demonstrating the need for comprehensive metabolite profiling in diagnosis. Nevertheless, the glucuronides of dihydrocortisol, dihydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and tetrahydrocortisone are the major ones. The sum of the glucuronidated and free forms constitute >93% of the metabolites analyzed, which is termed as total cortisol equivalent. Total cortisol equivalent may serve as a surrogate of cortisol secretion. Clinical trial registration number: NCT02500472</bold.
Original language | English (US) |
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Pages (from-to) | 2015-2026 |
Number of pages | 12 |
Journal | Bioanalysis |
Volume | 10 |
Issue number | 24 |
DOIs | |
State | Published - Dec 2018 |
Bibliographical note
Publisher Copyright:© 2018 Newlands Press.
Keywords
- cortisol metabolism
- high-resolution accurate-mass MS
- stress
- tobacco
- urine biomarker