Abstract
Sera from 35 patients diagnosed with human granulocytic ehrlichiosis in Connecticut were tested by indirect IFA staining methods with 5 strains of Ehrlichia equi or the human granulocytic ehrlichiosis agent to assess the suitability of different strains in laboratory analyses. Antigens included horse-derived infected neutrophils (MRK and BDS strains) and human isolates cultured in human promyelocytic leukemia cells (NCH-1, RCH, and Webster). Of 35 sera, 23 (65.7%) reacted to all 5 strains. Seropositivity was highest (97.1%) in assays that contained the MRK strain from California and lowest (71.4%) in tests with the NCH-1 strain from Nantucket, Massachusetts. In parallel testing of 32 sera with the NCH-1 strain by indirect IFA and Western blot analyses, results were concordant for 30 samples (93.8%). All strains of ehrlichiae can be used in IFA analyses for antibody detection, but assay sensitivity varied with the strain used.
Original language | English (US) |
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Pages (from-to) | 1835-1838 |
Number of pages | 4 |
Journal | Journal of Infectious Diseases |
Volume | 178 |
Issue number | 6 |
DOIs | |
State | Published - 1998 |
Externally published | Yes |
Bibliographical note
Funding Information:Financial support: CDC Emerging Infections Program (HR8/ CCH113382-01, U50/CCU111188-01), NIH (AI-41440), and federal Hatch funds. J.W.IJ. is a Daland Fellow of the American Philosophical Society.