Recombinant tri-segmented Pichinde virus as a novel live viral vaccine platform

Rekha Dhanwani; Hinh Ly; Yuying Liang

doi:10.1007/978-1-4939-6869-5_10

Recombinant tri-segmented Pichinde virus as a novel live viral vaccine platform

Rekha Dhanwani, Hinh Ly, Yuying Liang

Veterinary and Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

15 Scopus citations

Abstract

Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	169-179
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-6869-5_10
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1581
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported by National Institutes of HealthNIH grants R21AI094133 and R01AI083409 to Y.L., and R01AI093580 to H.L.

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Keywords

Arenavirus
Pichinde virus
Vaccine
Viral vaccine vector

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-6869-5_10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340294

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Cite this

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abstract = "Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.",

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AU - Ly, Hinh

AU - Liang, Yuying

N1 - Funding Information: This work was supported by National Institutes of HealthNIH grants R21AI094133 and R01AI083409 to Y.L., and R01AI093580 to H.L. Publisher Copyright: © Springer Science+Business Media LLC 2017.

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N2 - Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.

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KW - Vaccine

KW - Viral vaccine vector

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Recombinant tri-segmented Pichinde virus as a novel live viral vaccine platform

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

Access

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