TY - JOUR
T1 - Refined structures of three crystal forms of toxic shock syndrome toxin- 1 and of a tetramutant with reduced activity
AU - Prasad, G. Sridhar
AU - Radhakrishnan, Ramaswamy
AU - Mitchell, David T.
AU - Earhart, Cathleen A.
AU - Dinges, Martin M.
AU - Cook, William J.
AU - Schlievert, Patrick M.
AU - Ohlendorf, Douglas H.
PY - 1997/6
Y1 - 1997/6
N2 - The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 Å, 2.90 Å, and 2.75 Å, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry- related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1H at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, 1140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 Å resolution).
AB - The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 Å, 2.90 Å, and 2.75 Å, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry- related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1H at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, 1140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 Å resolution).
KW - Protein conformation
KW - Staphylococcus aureus
KW - Superantigens
KW - Toxins
KW - X-ray crystallography
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U2 - 10.1002/pro.5560060610
DO - 10.1002/pro.5560060610
M3 - Article
C2 - 9194182
AN - SCOPUS:0030958816
SN - 0961-8368
VL - 6
SP - 1220
EP - 1227
JO - Protein Science
JF - Protein Science
IS - 6
ER -
