Ribosomal s6 protein kinase 4: A prognostic factor for renal cell carcinoma

L. Fan; P. Li; Z. Yin; G. Fu; D. J. Liao; Y. Liu; J. Zhu; Y. Zhang; L. Wang; Q. Yan; Y. Guo; C. Shao; G. Huang; Z. Wang

doi:10.1038/bjc.2013.463

Ribosomal s6 protein kinase 4: A prognostic factor for renal cell carcinoma

L. Fan, P. Li, Z. Yin, G. Fu, D. J. Liao, Y. Liu, J. Zhu, Y. Zhang, L. Wang, Q. Yan, Y. Guo, C. Shao, G. Huang, Z. Wang

Hormel Institute

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background:The expression and function of ribosomal s6 protein kinase 4 (RSK4) in renal cell carcinoma (RCC) are unknown.Methods:Immunohistochemistry was used to detect the expression of RSK4 in RCC, and the relationship between RSK4 expression and clinicopathological features as well as prognosis of RCC patients was statistically analysed. Ectopic RSK4 expression in RCC cell lines was performed to determine its effect on cell cycle regulation, tumour invasiveness, and metastatic capability.Results:RSK4 was overexpressed in RCCs (P=0.003), compared with normal tissues, and the expression varied in different RCC subtypes (P=0.021), especially in two subtypes of papillary RCCs (P=0.001). RSK4 expression was positively correlated with high pT stage (P<0.001), high Fuhrman grade (P<0.001), lymph node involvement (P<0.001), and presence of distant metastasis (P=0.039), and could predict poor outcome in RCC patients. Molecular studies showed that overexpression of RSK4 could promote cell cycle progression and enhance the invasive and metastatic capability of RCC cell lines and vice versa.Conclusion:The expression pattern and molecular mechanisms of RSK4 in RCCs indicate that it could be a potential independent prognostic factor and serve as a new potential therapeutic target for RCC patients.

Original language	English (US)
Pages (from-to)	1137-1146
Number of pages	10
Journal	British Journal of Cancer
Volume	109
Issue number	5
DOIs	https://doi.org/10.1038/bjc.2013.463
State	Published - Sep 2013

Bibliographical note

Funding Information:
We thank Professor Cajal (Department of Pathology, Vall d’Hebron University Hospital, Barcelona, Spain) and Professor Jian Zhang (Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, China) for providing the human shRSK4 plasmids and the packaging plasmids. We are also grateful to the Department of Medical Records Xijing Hospital for excellent working conditions and for providing access to archival materials. The work is supported by the National Natural Science Foundation of China (No. 81001140 and No. 81272651).

Keywords

ribosomal s6 protein kinase 4; renal cell carcinoma; prognosis; invasion; metastasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1038/bjc.2013.463

OpenUrl availability

Full text

Cite this

@article{9bd499d025c54c6a8000a73ac0b90d13,

title = "Ribosomal s6 protein kinase 4: A prognostic factor for renal cell carcinoma",

abstract = "Background:The expression and function of ribosomal s6 protein kinase 4 (RSK4) in renal cell carcinoma (RCC) are unknown.Methods:Immunohistochemistry was used to detect the expression of RSK4 in RCC, and the relationship between RSK4 expression and clinicopathological features as well as prognosis of RCC patients was statistically analysed. Ectopic RSK4 expression in RCC cell lines was performed to determine its effect on cell cycle regulation, tumour invasiveness, and metastatic capability.Results:RSK4 was overexpressed in RCCs (P=0.003), compared with normal tissues, and the expression varied in different RCC subtypes (P=0.021), especially in two subtypes of papillary RCCs (P=0.001). RSK4 expression was positively correlated with high pT stage (P<0.001), high Fuhrman grade (P<0.001), lymph node involvement (P<0.001), and presence of distant metastasis (P=0.039), and could predict poor outcome in RCC patients. Molecular studies showed that overexpression of RSK4 could promote cell cycle progression and enhance the invasive and metastatic capability of RCC cell lines and vice versa.Conclusion:The expression pattern and molecular mechanisms of RSK4 in RCCs indicate that it could be a potential independent prognostic factor and serve as a new potential therapeutic target for RCC patients.",

keywords = "ribosomal s6 protein kinase 4; renal cell carcinoma; prognosis; invasion; metastasis",

author = "L. Fan and P. Li and Z. Yin and G. Fu and Liao, {D. J.} and Y. Liu and J. Zhu and Y. Zhang and L. Wang and Q. Yan and Y. Guo and C. Shao and G. Huang and Z. Wang",

note = "Funding Information: We thank Professor Cajal (Department of Pathology, Vall d{\textquoteright}Hebron University Hospital, Barcelona, Spain) and Professor Jian Zhang (Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi{\textquoteright}an, China) for providing the human shRSK4 plasmids and the packaging plasmids. We are also grateful to the Department of Medical Records Xijing Hospital for excellent working conditions and for providing access to archival materials. The work is supported by the National Natural Science Foundation of China (No. 81001140 and No. 81272651).",

year = "2013",

month = sep,

doi = "10.1038/bjc.2013.463",

language = "English (US)",

volume = "109",

pages = "1137--1146",

journal = "British Journal of Cancer",

issn = "0007-0920",

publisher = "Nature Publishing Group",

number = "5",

}

TY - JOUR

T1 - Ribosomal s6 protein kinase 4

T2 - A prognostic factor for renal cell carcinoma

AU - Fan, L.

AU - Li, P.

AU - Yin, Z.

AU - Fu, G.

AU - Liao, D. J.

AU - Liu, Y.

AU - Zhu, J.

AU - Zhang, Y.

AU - Wang, L.

AU - Yan, Q.

AU - Guo, Y.

AU - Shao, C.

AU - Huang, G.

AU - Wang, Z.

N1 - Funding Information: We thank Professor Cajal (Department of Pathology, Vall d’Hebron University Hospital, Barcelona, Spain) and Professor Jian Zhang (Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, China) for providing the human shRSK4 plasmids and the packaging plasmids. We are also grateful to the Department of Medical Records Xijing Hospital for excellent working conditions and for providing access to archival materials. The work is supported by the National Natural Science Foundation of China (No. 81001140 and No. 81272651).

PY - 2013/9

Y1 - 2013/9

N2 - Background:The expression and function of ribosomal s6 protein kinase 4 (RSK4) in renal cell carcinoma (RCC) are unknown.Methods:Immunohistochemistry was used to detect the expression of RSK4 in RCC, and the relationship between RSK4 expression and clinicopathological features as well as prognosis of RCC patients was statistically analysed. Ectopic RSK4 expression in RCC cell lines was performed to determine its effect on cell cycle regulation, tumour invasiveness, and metastatic capability.Results:RSK4 was overexpressed in RCCs (P=0.003), compared with normal tissues, and the expression varied in different RCC subtypes (P=0.021), especially in two subtypes of papillary RCCs (P=0.001). RSK4 expression was positively correlated with high pT stage (P<0.001), high Fuhrman grade (P<0.001), lymph node involvement (P<0.001), and presence of distant metastasis (P=0.039), and could predict poor outcome in RCC patients. Molecular studies showed that overexpression of RSK4 could promote cell cycle progression and enhance the invasive and metastatic capability of RCC cell lines and vice versa.Conclusion:The expression pattern and molecular mechanisms of RSK4 in RCCs indicate that it could be a potential independent prognostic factor and serve as a new potential therapeutic target for RCC patients.

AB - Background:The expression and function of ribosomal s6 protein kinase 4 (RSK4) in renal cell carcinoma (RCC) are unknown.Methods:Immunohistochemistry was used to detect the expression of RSK4 in RCC, and the relationship between RSK4 expression and clinicopathological features as well as prognosis of RCC patients was statistically analysed. Ectopic RSK4 expression in RCC cell lines was performed to determine its effect on cell cycle regulation, tumour invasiveness, and metastatic capability.Results:RSK4 was overexpressed in RCCs (P=0.003), compared with normal tissues, and the expression varied in different RCC subtypes (P=0.021), especially in two subtypes of papillary RCCs (P=0.001). RSK4 expression was positively correlated with high pT stage (P<0.001), high Fuhrman grade (P<0.001), lymph node involvement (P<0.001), and presence of distant metastasis (P=0.039), and could predict poor outcome in RCC patients. Molecular studies showed that overexpression of RSK4 could promote cell cycle progression and enhance the invasive and metastatic capability of RCC cell lines and vice versa.Conclusion:The expression pattern and molecular mechanisms of RSK4 in RCCs indicate that it could be a potential independent prognostic factor and serve as a new potential therapeutic target for RCC patients.

KW - ribosomal s6 protein kinase 4; renal cell carcinoma; prognosis; invasion; metastasis

UR - http://www.scopus.com/inward/record.url?scp=84883739890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883739890&partnerID=8YFLogxK

U2 - 10.1038/bjc.2013.463

DO - 10.1038/bjc.2013.463

M3 - Article

C2 - 23942078

AN - SCOPUS:84883739890

SN - 0007-0920

VL - 109

SP - 1137

EP - 1146

JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 5

ER -

Ribosomal s6 protein kinase 4: A prognostic factor for renal cell carcinoma

Abstract

Bibliographical note

Keywords

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this