Abstract
A variety of cultured cell types are effectively killed by Ca2 +-carrying ionophores (A23187, ionomycin and lasalocid) in the presence of adequate extracellular concentrations of Ca2 + and Na +, although cell killing mechanisms independent of these ions also exist. Previous studies identified 2 injury stages (termed stage I and stage II injury) at which the A23187-induced killing process in 3T3 fibroblasts is interrupted in the presence of low extracellular Ca2 + concentrations and in the absence of extracellular Na +, respectively. The present studies confirm the generality of stage II injury in Ca2 + -mediated cell killing, but demonstrate a requirement for concomitant stress conditions (e.g., osmotic or oxidative stress) for expression of stage I injury.
Original language | English (US) |
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Pages (from-to) | 283-293 |
Number of pages | 11 |
Journal | Toxicology Letters |
Volume | 39 |
Issue number | 2-3 |
DOIs | |
State | Published - Dec 1987 |
Keywords
- Cytotoxicity
- ionomycin
- ionophore A23187
- lasalocid
- osmotic stress
- oxidative stress