Secretion of heterologous cyclodextrin glycosyltransferase of bacillus sp. E1 from escherichia coli

Jeongsik Yong; Jin Nam Choi; Sung Soon Park; Cheon Seok Park; Kwan Hwa Park; Yang Do Choi

doi:10.1007/BF00128597

Secretion of heterologous cyclodextrin glycosyltransferase of bacillus sp. E1 from escherichia coli

Jeongsik Yong, Jin Nam Choi, Sung Soon Park, Cheon Seok Park, Kwan Hwa Park, Yang Do Choi

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

To characterize the molecular properties of CGTase from alkalophilic Bacillus sp. E1 (BCGTE1), a genomic clone for a CGTase was isolated. Expression of recombinant BCGTE1 in E. coil was analyzed by immunoblotting. It showed that the nascent recombinant BCGTE1 expressed was 87 kDa but it was processed into the mature enzyme of 81 kDa. With the process it was secreted predominantly into the culture medium via periplasmic space. This feature is different from other Bacillus CGTases expressed in E. coil, which were present mostly in the periplasmic space.

Original language	English (US)
Pages (from-to)	1223-1228
Number of pages	6
Journal	Biotechnology Letters
Volume	18
Issue number	10
DOIs	https://doi.org/10.1007/BF00128597
State	Published - 1996

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title = "Secretion of heterologous cyclodextrin glycosyltransferase of bacillus sp. E1 from escherichia coli",

abstract = "To characterize the molecular properties of CGTase from alkalophilic Bacillus sp. E1 (BCGTE1), a genomic clone for a CGTase was isolated. Expression of recombinant BCGTE1 in E. coil was analyzed by immunoblotting. It showed that the nascent recombinant BCGTE1 expressed was 87 kDa but it was processed into the mature enzyme of 81 kDa. With the process it was secreted predominantly into the culture medium via periplasmic space. This feature is different from other Bacillus CGTases expressed in E. coil, which were present mostly in the periplasmic space.",

author = "Jeongsik Yong and Choi, {Jin Nam} and Park, {Sung Soon} and Park, {Cheon Seok} and Park, {Kwan Hwa} and Choi, {Yang Do}",

year = "1996",

doi = "10.1007/BF00128597",

language = "English (US)",

volume = "18",

pages = "1223--1228",

journal = "Biotechnology Letters",

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T1 - Secretion of heterologous cyclodextrin glycosyltransferase of bacillus sp. E1 from escherichia coli

AU - Yong, Jeongsik

AU - Choi, Jin Nam

AU - Park, Sung Soon

AU - Park, Cheon Seok

AU - Park, Kwan Hwa

AU - Choi, Yang Do

PY - 1996

Y1 - 1996

N2 - To characterize the molecular properties of CGTase from alkalophilic Bacillus sp. E1 (BCGTE1), a genomic clone for a CGTase was isolated. Expression of recombinant BCGTE1 in E. coil was analyzed by immunoblotting. It showed that the nascent recombinant BCGTE1 expressed was 87 kDa but it was processed into the mature enzyme of 81 kDa. With the process it was secreted predominantly into the culture medium via periplasmic space. This feature is different from other Bacillus CGTases expressed in E. coil, which were present mostly in the periplasmic space.

AB - To characterize the molecular properties of CGTase from alkalophilic Bacillus sp. E1 (BCGTE1), a genomic clone for a CGTase was isolated. Expression of recombinant BCGTE1 in E. coil was analyzed by immunoblotting. It showed that the nascent recombinant BCGTE1 expressed was 87 kDa but it was processed into the mature enzyme of 81 kDa. With the process it was secreted predominantly into the culture medium via periplasmic space. This feature is different from other Bacillus CGTases expressed in E. coil, which were present mostly in the periplasmic space.

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Secretion of heterologous cyclodextrin glycosyltransferase of bacillus sp. E1 from escherichia coli

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