Abstract
(Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).
Original language | English (US) |
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Pages (from-to) | 10179-10182 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 47 |
Issue number | 52 |
DOIs | |
State | Published - Dec 15 2008 |
Keywords
- Antibiotics
- Antibodies
- Diabodies
- Macrocyclization
- Self-assembly