Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme

Youlin Xia; Xiangming Kong; David K. Smith; Yu Liu; David Man; Guang Zhu

doi:10.1006/jmre.2000.2030

Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme

Youlin Xia, Xiangming Kong, David K. Smith, Yu Liu, David Man, Guang Zhu

Minnesota NMR Center

Research output: Contribution to journal › Editorial › peer-review

10 Scopus citations

Abstract

Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of ¹ H ^α - ¹³ C ^α (R _MQ ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of ¹³ C ^α (R _C ) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D ₂ O at 20°C.

Original language	English (US)
Pages (from-to)	407-410
Number of pages	4
Journal	Journal of Magnetic Resonance
Volume	143
Issue number	2
DOIs	https://doi.org/10.1006/jmre.2000.2030
State	Published - Apr 2000

Bibliographical note

Funding Information:
This work is supported by grants (HKUST6197/97M and HKUST6038/ 98M) from the Research Grant Council of Hong Kong. We thank Dr. K. H. Sze for critical discussions. The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 500-MHz NMR spectrometer.

Keywords

C-labeled proteins
HCACO
HMQC
NMR

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title = "Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme",

abstract = " Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of 1 H α - 13 C α (R MQ ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of 13 C α (R C ) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D 2 O at 20°C.",

keywords = "C-labeled proteins, HCACO, HMQC, NMR",

author = "Youlin Xia and Xiangming Kong and Smith, {David K.} and Yu Liu and David Man and Guang Zhu",

note = "Funding Information: This work is supported by grants (HKUST6197/97M and HKUST6038/ 98M) from the Research Grant Council of Hong Kong. We thank Dr. K. H. Sze for critical discussions. The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 500-MHz NMR spectrometer.",

year = "2000",

month = apr,

doi = "10.1006/jmre.2000.2030",

language = "English (US)",

volume = "143",

pages = "407--410",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

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T1 - Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme

AU - Xia, Youlin

AU - Kong, Xiangming

AU - Smith, David K.

AU - Liu, Yu

AU - Man, David

AU - Zhu, Guang

N1 - Funding Information: This work is supported by grants (HKUST6197/97M and HKUST6038/ 98M) from the Research Grant Council of Hong Kong. We thank Dr. K. H. Sze for critical discussions. The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 500-MHz NMR spectrometer.

PY - 2000/4

Y1 - 2000/4

N2 - Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of 1 H α - 13 C α (R MQ ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of 13 C α (R C ) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D 2 O at 20°C.

AB - Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of 1 H α - 13 C α (R MQ ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of 13 C α (R C ) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D 2 O at 20°C.

KW - C-labeled proteins

KW - HCACO

KW - HMQC

KW - NMR

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U2 - 10.1006/jmre.2000.2030

DO - 10.1006/jmre.2000.2030

M3 - Editorial

C2 - 10729268

AN - SCOPUS:0034167528

SN - 1090-7807

VL - 143

SP - 407

EP - 410

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

IS - 2

ER -

Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme

Abstract

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Keywords

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