Sequential G- to R-banding for high resolution chromosome analysis

Betsy Hirsch; Rosalie Mack; Diane Arthur

doi:10.1007/BF00283047

Sequential G- to R-banding for high resolution chromosome analysis

Betsy Hirsch, Rosalie Mack, Diane Arthur

Laboratory Medicine and Pathology

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5 Scopus citations

Abstract

A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

Original language	English (US)
Pages (from-to)	37-39
Number of pages	3
Journal	Human Genetics
Volume	76
Issue number	1
DOIs	https://doi.org/10.1007/BF00283047
State	Published - May 1987

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title = "Sequential G- to R-banding for high resolution chromosome analysis",

abstract = "A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.",

author = "Betsy Hirsch and Rosalie Mack and Diane Arthur",

year = "1987",

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doi = "10.1007/BF00283047",

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AU - Hirsch, Betsy

AU - Mack, Rosalie

AU - Arthur, Diane

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N2 - A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

AB - A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

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Sequential G- to R-banding for high resolution chromosome analysis

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