Abstract
A simple procedure for simultaneously derivatizing non-hydroxy and hydroxy fatty acids prior to GC analysis [I. Ciucanu and F. Kerek, J. Chromatogr., 284 (1984) 179] has been evaluated for its usefulness in determining sphingolipid acyl composition. The method uses methyl iodide in polar aprotic solvents to generate methyl esters of carboxyl groups and methyl ethers of hydroxyl groups. Methylation efficiency is examined as a function of hydroxyl group presence and location in free fatty acids as well as a function of 2-hydroxy fatty acid chain length. Conditions are also reported for efficient saponification and derivatization of sphingolipid fatty acyl chains as is illustrated using bovine brain galactosylceramide.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 281-286 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 605 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 17 1992 |
Bibliographical note
Funding Information:We would like to thank Dr. Shaukat Ali for synthesizing the sphingolipid internal standards, Drs. Hermann Schlenk and Herb Dutton for their helpful comments, Carmen Perleberg for secretarial services, and Core D of USPHS PPG HL08214 for the use of GC-MS equipment. This work was supported by USPHS Grant GM45928 and by the Hormel Foundation.