Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Bradley J. Segura; Weizhen Zhang; Liqun Xiao; Craig D. Logsdon; Michael W. Mulholland

doi:10.1016/j.molbrainres.2004.10.016

Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Bradley J. Segura, Weizhen Zhang, Liqun Xiao, Craig D. Logsdon, Michael W. Mulholland

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Abstract

Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

Original language	English (US)
Pages (from-to)	325-328
Number of pages	4
Journal	Molecular Brain Research
Volume	133
Issue number	2
DOIs	https://doi.org/10.1016/j.molbrainres.2004.10.016
State	Published - Feb 18 2005

Bibliographical note

Funding Information:
This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.

Keywords

C-fos
Early response gene
Glia
Lysophosphatidic acid
Sphingosine-1-phosphate
Sphingosylphosphorylcholine

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells",

abstract = "Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.",

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author = "Segura, {Bradley J.} and Weizhen Zhang and Liqun Xiao and Logsdon, {Craig D.} and Mulholland, {Michael W.}",

note = "Funding Information: This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.",

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AU - Segura, Bradley J.

AU - Zhang, Weizhen

AU - Xiao, Liqun

AU - Logsdon, Craig D.

AU - Mulholland, Michael W.

N1 - Funding Information: This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.

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AB - Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

KW - C-fos

KW - Early response gene

KW - Glia

KW - Lysophosphatidic acid

KW - Sphingosine-1-phosphate

KW - Sphingosylphosphorylcholine

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Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Abstract

Bibliographical note

Keywords

UN SDGs

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