TY - JOUR
T1 - Stem cells for myocardial repair with use of a transarterial catheter
AU - Wang, Xiaohong
AU - Jameel, Mohammad Nurulqadr
AU - Li, Qinglu
AU - Mansoor, Abdul
AU - Qiang, Xiong
AU - Swingen, Cory M
AU - Panetta, Carmelo
AU - Zhang, Jianyi J
PY - 2009
Y1 - 2009
N2 - Background-Using a swine model of postinfarction left ventricle (LV) remodeling, we investigated marrow-derived, multipotent progenitor cell (MPC) transplantation into hearts with acute myocardial infarction (AMI) via a novel transarterial catheter. Methods and Results-The left anterior descending coronary artery was balloon-occluded after percutaneous transluminal angiography to generate AMI (60-minute no-flow ischemia). The transarterial catheter was then placed in the same coronary artery, and either 50X106 MPCs (cell group, n=6) or saline (control, n=6) was injected into the border zone (BZ) myocardium. LV function was assessed by magnetic resonance imaging before AMI and at 1 and 4 weeks after AMI, whereas myocardial energy metabolism was assessed by 31P-magnetic resonance spectroscopy at week 4. One week after AMI, the ejection fraction was significantly reduced in both groups from a baseline of ≈50% to 31.3 ±3.9% (cell group) and 33.3 ±3.1% (control). However, at week 4, the cell group had a significant recovery in ejection fraction. The functional improvements were accompanied by a significant improvement in myocardial bioenergetics. Histologic data demonstrated a 0.55% cell engraftment rate 4 weeks after MPC transplantation. Only 2% of engrafted cells were costaining positive for cardiogenic markers. Vascular density in the BZ was increased in the cell group. Conditioned medium from cultured MPCs contained high levels of vascular endothelial growth factor, which was increased in response to hypoxia. MPCs cocultured with cardiomyocytes inhibited changes in cardiomyocyte mitochondrial membrane potential and cytochrome c release induced by tumor necrosis factor-a. Conclusions-Thus, a paracrine effect may contribute significantly to the observed therapeutic effects of MPC transplantation.
AB - Background-Using a swine model of postinfarction left ventricle (LV) remodeling, we investigated marrow-derived, multipotent progenitor cell (MPC) transplantation into hearts with acute myocardial infarction (AMI) via a novel transarterial catheter. Methods and Results-The left anterior descending coronary artery was balloon-occluded after percutaneous transluminal angiography to generate AMI (60-minute no-flow ischemia). The transarterial catheter was then placed in the same coronary artery, and either 50X106 MPCs (cell group, n=6) or saline (control, n=6) was injected into the border zone (BZ) myocardium. LV function was assessed by magnetic resonance imaging before AMI and at 1 and 4 weeks after AMI, whereas myocardial energy metabolism was assessed by 31P-magnetic resonance spectroscopy at week 4. One week after AMI, the ejection fraction was significantly reduced in both groups from a baseline of ≈50% to 31.3 ±3.9% (cell group) and 33.3 ±3.1% (control). However, at week 4, the cell group had a significant recovery in ejection fraction. The functional improvements were accompanied by a significant improvement in myocardial bioenergetics. Histologic data demonstrated a 0.55% cell engraftment rate 4 weeks after MPC transplantation. Only 2% of engrafted cells were costaining positive for cardiogenic markers. Vascular density in the BZ was increased in the cell group. Conditioned medium from cultured MPCs contained high levels of vascular endothelial growth factor, which was increased in response to hypoxia. MPCs cocultured with cardiomyocytes inhibited changes in cardiomyocyte mitochondrial membrane potential and cytochrome c release induced by tumor necrosis factor-a. Conclusions-Thus, a paracrine effect may contribute significantly to the observed therapeutic effects of MPC transplantation.
KW - Metabolism
KW - Myocardial infarct
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=70349787215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349787215&partnerID=8YFLogxK
U2 - 10.1161/CIRCULATIONAHA.109.885236
DO - 10.1161/CIRCULATIONAHA.109.885236
M3 - Article
C2 - 19752374
AN - SCOPUS:70349787215
SN - 0009-7322
VL - 120
SP - S238-S246
JO - Circulation
JF - Circulation
IS - SUPPL. 1
ER -