TY - CHAP
T1 - Streak Fluorescence Lifetime Imaging Microscopy. A Novel Technology for Quantitative FRET Imaging
AU - Ramanujan, V. Krishnan
AU - Zhang, Jian Hua
AU - Centonze, Victoria E.
AU - Herman, Brian
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - This chapter provides a comprehensive description of this streak camera-based fluorescence lifetime imaging microscopy (FLIM) imaging (StreakFLIM) system and contrasts its performance with that of the other currently available time-domain FLIM systems. The FLIM methods are relatively insensitive to artifacts that can affect spectroscopic characteristics of fluorophores and can provide distinct lifetimes characteristic of the individual fluorophores attached to each protein (for example, donor and acceptor). The main component of the StreakFLIM system is the streak camera, also known as streakscope, a device that can measure ultrafast light phenomena with a temporal resolution as low as 2 picoseconds (ps). In addition to intensity and time information, the streakscope can be used to obtain either spatial or wavelength information. The streak camera has good radiant sensitivity over a wide bandwidth of wavelengths, making it suitable for both single-photon and multiphoton imaging applications without compromising detection sensitivity. While in the conventional time-domain FLIM, multigate detection with a gated microchannel plate image intensifier and a charge-coupled device (CCD) imaging camera is used.
AB - This chapter provides a comprehensive description of this streak camera-based fluorescence lifetime imaging microscopy (FLIM) imaging (StreakFLIM) system and contrasts its performance with that of the other currently available time-domain FLIM systems. The FLIM methods are relatively insensitive to artifacts that can affect spectroscopic characteristics of fluorophores and can provide distinct lifetimes characteristic of the individual fluorophores attached to each protein (for example, donor and acceptor). The main component of the StreakFLIM system is the streak camera, also known as streakscope, a device that can measure ultrafast light phenomena with a temporal resolution as low as 2 picoseconds (ps). In addition to intensity and time information, the streakscope can be used to obtain either spatial or wavelength information. The streak camera has good radiant sensitivity over a wide bandwidth of wavelengths, making it suitable for both single-photon and multiphoton imaging applications without compromising detection sensitivity. While in the conventional time-domain FLIM, multigate detection with a gated microchannel plate image intensifier and a charge-coupled device (CCD) imaging camera is used.
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U2 - 10.1016/B978-019517720-6.50021-3
DO - 10.1016/B978-019517720-6.50021-3
M3 - Chapter
AN - SCOPUS:34548233655
SN - 9780195177206
SP - 227
EP - 238
BT - Molecular Imaging
PB - Elsevier Inc.
ER -