Structural characterization and antiviral activity of a novel heteropolysaccharide isolated from Grifola frondosa against enterovirus 71

Chao Zhao; Luying Gao; Chunyang Wang; Bin Liu; Yu Jin; Zheng Xing

doi:10.1016/j.carbpol.2015.12.005

Structural characterization and antiviral activity of a novel heteropolysaccharide isolated from Grifola frondosa against enterovirus 71

Chao Zhao, Luying Gao, Chunyang Wang, Bin Liu, Yu Jin, Zheng Xing

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

A novel heteropolysaccharide from Grifola frondosa mycelia was extracted and purified using DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (¹H NMR and ¹³C NMR) spectroscopy were used to decipher the structure of the purified G. frondosa polysaccharide (GFP1). Chemical and spectral analysis revealed that GFP1, with an average molecular weight of 40.5 kDa, possessed a 1,6-β-d-glucan backbone with a single 1,3-α-d-fucopyranosyl side-branching unit. Enterovirus 71 (EV71) is the causative pathogen of hand-foot-and-mouth disease. GFP1 was tested for its anti-EV71 activity in cultured cells, which showed that EV71 viral replication was blocked and viral VP1 protein expression and genomic RNA synthesis were suppressed. Moreover, GFP1 exhibited apoptotic and other activities by suppressing the EV71-induced caspase-3 cleavage and IκBα down regulation. Our results demonstrate that the novel G. frondosa polysaccharide has antiviral activity, which could be valuable as a potentially new anti-EV71 therapeutic compound.

Original language	English (US)
Pages (from-to)	382-389
Number of pages	8
Journal	Carbohydrate Polymers
Volume	144
DOIs	https://doi.org/10.1016/j.carbpol.2015.12.005
State	Published - Jun 25 2016

Bibliographical note

Funding Information:
This study was financially supported by the School-Enterprise Cooperation Major Projects of Fujian, China ( 2014N5007 ) to CZ and BL, FAFU grants ( XJQ201417 & 612014042 ) to CZ, and the Natural Science Foundation of Jiangsu Province, China ( BK20141078 ) to YJ. The project was also supported by the Mega Infectious Diseases Program from the Ministry of Science of Technology of China ( 2014ZX10004001-002 ) and a grant from the State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University ( KFGW-200902 ) to ZX.

Publisher Copyright:
© 2016 Published by Elsevier Ltd.

Keywords

Antiviral
Chemical properties
Enterovirus 71
Grifola frondosa polysaccharide
Viral replication

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title = "Structural characterization and antiviral activity of a novel heteropolysaccharide isolated from Grifola frondosa against enterovirus 71",

abstract = "A novel heteropolysaccharide from Grifola frondosa mycelia was extracted and purified using DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (1H NMR and 13C NMR) spectroscopy were used to decipher the structure of the purified G. frondosa polysaccharide (GFP1). Chemical and spectral analysis revealed that GFP1, with an average molecular weight of 40.5 kDa, possessed a 1,6-β-d-glucan backbone with a single 1,3-α-d-fucopyranosyl side-branching unit. Enterovirus 71 (EV71) is the causative pathogen of hand-foot-and-mouth disease. GFP1 was tested for its anti-EV71 activity in cultured cells, which showed that EV71 viral replication was blocked and viral VP1 protein expression and genomic RNA synthesis were suppressed. Moreover, GFP1 exhibited apoptotic and other activities by suppressing the EV71-induced caspase-3 cleavage and IκBα down regulation. Our results demonstrate that the novel G. frondosa polysaccharide has antiviral activity, which could be valuable as a potentially new anti-EV71 therapeutic compound.",

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author = "Chao Zhao and Luying Gao and Chunyang Wang and Bin Liu and Yu Jin and Zheng Xing",

note = "Funding Information: This study was financially supported by the School-Enterprise Cooperation Major Projects of Fujian, China ( 2014N5007 ) to CZ and BL, FAFU grants ( XJQ201417 & 612014042 ) to CZ, and the Natural Science Foundation of Jiangsu Province, China ( BK20141078 ) to YJ. The project was also supported by the Mega Infectious Diseases Program from the Ministry of Science of Technology of China ( 2014ZX10004001-002 ) and a grant from the State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University ( KFGW-200902 ) to ZX. Publisher Copyright: {\textcopyright} 2016 Published by Elsevier Ltd.",

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AU - Jin, Yu

AU - Xing, Zheng

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Structural characterization and antiviral activity of a novel heteropolysaccharide isolated from Grifola frondosa against enterovirus 71

Abstract

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Keywords

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