TY - JOUR
T1 - Substrate Specificity of the Arabidopsis thaliana Sucrose Transporter AtSUC2
AU - Chandran, Divya
AU - Reinders, Anke
AU - Ward, John M.
PY - 2003/11/7
Y1 - 2003/11/7
N2 - The Arabidopsis sucrose transporter AtSUC2 is expressed in the companion cells of the phloem (specialized vascular tissue) and is essential for the long distance transport of carbohydrates within the plant. A variety of glucosides are known to inhibit sucrose uptake into yeast expressing AtSUC2; however, it remains unknown whether glucosides other than sucrose could serve as transported substrates. By expression of AtSUC2 in Xenopus oocytes and two-electrode voltage clamping, we have tested the ability of AtSUC2 to transport a range of physiological and synthetic glucosides. Sucrose induced inward currents with a K0.5 of 1.44 mM at pH 5 and a membrane potential of -137 mV. Of the 24 additional sugars tested, 8 glucosides induced large inward currents allowing kinetic analysis. These glucosides were maltose, arbutin (hydroquinone-β-D-glucoside), salicin (2-(hydroxymethyl)phenyl-β-D-glucoside), α-phenylglucoside, β-phenylglucoside, α-paranitrophenylglucoside, β -paranitrophenylglucoside, and paranitrophenyl-β-thioglucoside. In addition, turanose and α-methylglucoside induced small but significant inward currents indicating that they were transported by AtSUC2. The results indicate that AtSUC2 is not highly selective for α- over β-glucosides and may function in transporting glucosides besides sucrose into the phloem, and the results provide insight into the structural requirements for transport by AtSUC2.
AB - The Arabidopsis sucrose transporter AtSUC2 is expressed in the companion cells of the phloem (specialized vascular tissue) and is essential for the long distance transport of carbohydrates within the plant. A variety of glucosides are known to inhibit sucrose uptake into yeast expressing AtSUC2; however, it remains unknown whether glucosides other than sucrose could serve as transported substrates. By expression of AtSUC2 in Xenopus oocytes and two-electrode voltage clamping, we have tested the ability of AtSUC2 to transport a range of physiological and synthetic glucosides. Sucrose induced inward currents with a K0.5 of 1.44 mM at pH 5 and a membrane potential of -137 mV. Of the 24 additional sugars tested, 8 glucosides induced large inward currents allowing kinetic analysis. These glucosides were maltose, arbutin (hydroquinone-β-D-glucoside), salicin (2-(hydroxymethyl)phenyl-β-D-glucoside), α-phenylglucoside, β-phenylglucoside, α-paranitrophenylglucoside, β -paranitrophenylglucoside, and paranitrophenyl-β-thioglucoside. In addition, turanose and α-methylglucoside induced small but significant inward currents indicating that they were transported by AtSUC2. The results indicate that AtSUC2 is not highly selective for α- over β-glucosides and may function in transporting glucosides besides sucrose into the phloem, and the results provide insight into the structural requirements for transport by AtSUC2.
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U2 - 10.1074/jbc.M308490200
DO - 10.1074/jbc.M308490200
M3 - Article
C2 - 12954621
AN - SCOPUS:0242666390
SN - 0021-9258
VL - 278
SP - 44320
EP - 44325
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 45
ER -