TY - JOUR
T1 - Susceptibility of ducks to avian pneumovirus of turkey origin
AU - Shin, Hyun Jin
AU - Njenga, Moses K.
AU - Halvorson, David A.
AU - Shaw, Daniel P.
AU - Nagaraja, Kakambi V.
PY - 2001/7
Y1 - 2001/7
N2 - Objective - To determine the susceptibility of ducks to avian pneumovirus (APV) of turkey origin. Animals - 30 Pekin ducks that were 2 weeks old. Procedure - Ducks were assigned to 3 groups (10 ducks/group). Ducks of groups 1 and 2 were inoculated (day 0) with 200 μl of cell-culture fluid containing APV of turkey origin (1055 median tissue-culture infective dose/ml) by the oculonasal (group 1) or oral (group 2) route. Ducks of group 3 served as noninoculated control birds. Two ducks from each group were euthanatized 3, 6, 9, 15, and 21 days after inoculation. Blood samples, tissue samples from the lungs, trachea, nasal turbinates, duodenum, diverticulum vitellinum (Meckel's diverticulum), and cecum, and swab specimens from the choana, cloaca, and trachea were obtained from all birds during necropsy and examined for APV by use of reverse transcriptase-polymerase chain reaction (RT-PCR), virus isolation, and histologic examination. Blood samples also were examined for APV antibodies, using an ELISA. Results - Tissue samples obtained up to 21 days after inoculation had positive results when tested by use of RT-PCR. Virus was isolated from nasal turbinates of birds inoculated via the oculonasal route. Serum samples obtained 15 and 21 days after inoculation had positive results when tested for APV-specific antibody. Clinical signs of disease were not observed in ducks inoculated with APV of turkey origin. Conclusions and Clinical Relevance - Ducks inoculated with APV of turkey origin may not develop clinical signs of disease, but they are suspected to play a role as nonclinical carriers of APV.
AB - Objective - To determine the susceptibility of ducks to avian pneumovirus (APV) of turkey origin. Animals - 30 Pekin ducks that were 2 weeks old. Procedure - Ducks were assigned to 3 groups (10 ducks/group). Ducks of groups 1 and 2 were inoculated (day 0) with 200 μl of cell-culture fluid containing APV of turkey origin (1055 median tissue-culture infective dose/ml) by the oculonasal (group 1) or oral (group 2) route. Ducks of group 3 served as noninoculated control birds. Two ducks from each group were euthanatized 3, 6, 9, 15, and 21 days after inoculation. Blood samples, tissue samples from the lungs, trachea, nasal turbinates, duodenum, diverticulum vitellinum (Meckel's diverticulum), and cecum, and swab specimens from the choana, cloaca, and trachea were obtained from all birds during necropsy and examined for APV by use of reverse transcriptase-polymerase chain reaction (RT-PCR), virus isolation, and histologic examination. Blood samples also were examined for APV antibodies, using an ELISA. Results - Tissue samples obtained up to 21 days after inoculation had positive results when tested by use of RT-PCR. Virus was isolated from nasal turbinates of birds inoculated via the oculonasal route. Serum samples obtained 15 and 21 days after inoculation had positive results when tested for APV-specific antibody. Clinical signs of disease were not observed in ducks inoculated with APV of turkey origin. Conclusions and Clinical Relevance - Ducks inoculated with APV of turkey origin may not develop clinical signs of disease, but they are suspected to play a role as nonclinical carriers of APV.
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U2 - 10.2460/ajvr.2001.62.991
DO - 10.2460/ajvr.2001.62.991
M3 - Article
C2 - 11453499
AN - SCOPUS:0035409413
SN - 0002-9645
VL - 62
SP - 991
EP - 994
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 7
ER -