TY - JOUR
T1 - Synergistic effects of N-ethyl-N-Nitrosourea (an alkylating agent with a low Swain-Scott substrate constant) and x-rays in the stamen hairs of Tradescantia clone BNL 4430
AU - Shima, Naoko
AU - Ichikawa, Sadao
PY - 1997/5/29
Y1 - 1997/5/29
N2 - The mutagenic interaction between N-ethyl-N-nitrosourea (ENU) and X-rays was tested in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. ENU, a monofunctional alkylating agent with a low Swain-Scott substrate constant (s) of 0.26, exhibited a strong cytotoxicity. ENU-induced somatic pink mutation frequency per 104 hair-cell divisions increased with increasing ENU dose, with a slope of 1.243 on a log-log graph, the slope value being similar to that for X-ray-induced mutation frequency. Three out of five combined treatments with ENU and X-rays produced mutation frequencies significantly higher than those expected from the additive effects of the two mutagens. Clear synergistic effects were detected when relatively higher X-ray doses were applied, resembling those confirmed earlier between methyl methanesulfonate (MMS) and X-rays, although the s value for ENU is very much smaller than that (0.88) for MMS. It is therefore concluded that mutagenic interactions between alkylating agents and X-rays do not have any clear relationship with the s valves.
AB - The mutagenic interaction between N-ethyl-N-nitrosourea (ENU) and X-rays was tested in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. ENU, a monofunctional alkylating agent with a low Swain-Scott substrate constant (s) of 0.26, exhibited a strong cytotoxicity. ENU-induced somatic pink mutation frequency per 104 hair-cell divisions increased with increasing ENU dose, with a slope of 1.243 on a log-log graph, the slope value being similar to that for X-ray-induced mutation frequency. Three out of five combined treatments with ENU and X-rays produced mutation frequencies significantly higher than those expected from the additive effects of the two mutagens. Clear synergistic effects were detected when relatively higher X-ray doses were applied, resembling those confirmed earlier between methyl methanesulfonate (MMS) and X-rays, although the s value for ENU is very much smaller than that (0.88) for MMS. It is therefore concluded that mutagenic interactions between alkylating agents and X-rays do not have any clear relationship with the s valves.
KW - N-ethyl-N-nitrosourea
KW - Substrate constant
KW - Swain-Scott
KW - Synergistic effect
KW - Tradescantio stamen hairs
KW - X-rays
UR - http://www.scopus.com/inward/record.url?scp=0030905208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030905208&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1098-2280(1997)29:3<323::AID-EM13>3.0.CO;2-G
DO - 10.1002/(SICI)1098-2280(1997)29:3<323::AID-EM13>3.0.CO;2-G
M3 - Article
C2 - 9142176
AN - SCOPUS:0030905208
SN - 0893-6692
VL - 29
SP - 323
EP - 329
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
IS - 3
ER -